3.01 Cell Cycle Lab Report
Safety Notes:
Always handle microscopes and glass slides carefully.
Wash your hands after handling the prepared specimens.
Materials:
Compound light microscope
Glass microscope slide with prepared onion root tip specimen
Purpose:
understand and identify the stages of the cell cycle and mitosis. apply an analytical technique to estimate the relative length of each stage of the cell cycle.
Hypothesis:
What do you predict you will find about the number of cells in each of the phases of the cell cycle?
Provide an explanation for your prediction.
Procedure:
Data and Observations:
Create a data table containing a tally of the number of cells observed in each of the following stages:
Stage Number of Cells in Part 1 Number of Cells in Part 2
Interphase
Prophase
Metaphase
Anaphase
Telophase
Cytokinesis
Record any observations about the cells you observed (what does the cell look like for each stage):
Data Analysis:
Calculate the percentage of the cell cycle spent in each stage.
Number of cells in given stage ÷ total number of cells counted × 100 = % of the cell cycle spent in this stage
Create a graph that represents the time spent in each stage of the cell cycle.
Conclusion:
Be sure to answer the following reflection questions in the conclusion of your lab report:
Based on your data, what can you infer about the length of time spent in each stage of the cell cycle?
What stages were the longest and shortest? Give a brief explanation of why these stages may have that time period.
What is a distinguishing visible feature of each stage of the cell cycle?
What differences can you see when you compare the nucleus of a dividing cell with that of a nondividing cell?
If your observation had not been restricted to the tip of the onion root, how would the results be different?
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