Preview

Gram Staining

Satisfactory Essays
Open Document
Open Document
327 Words
Grammar
Grammar
Plagiarism
Plagiarism
Writing
Writing
Score
Score
Gram Staining
Gram Staining

Gram Staining is a method of differentiating bacterial species into two large groups (Gram-positive and Gram-negative). Gram Staining is a way to separate one large group of bacteria into two. Crystal violet is used to dye the cells, which is the primary stain. Those that retain the color are grouped as Gram-positive, and those that do not retain the color are grouped as Gram-negative. Many of the Gram-negative bacteria are pathogenic, making this process useful for detecting infections.

Materials 1) Microscope 2) Lab Coat 3) Gram Staining Lab 4) 2 Slides 5) Gram Stain Solution 6) Gram crystal violet 7) Gram iodine 8) Burner 9) Alcohol 10) Water 11) Staphylococcus epidermidis 12) Escherichia coli 13) Bacillus subtillis 14) Gram Safrain 15)
Procedures
First I get all my materials to get started. I make sure I turn on the burner and sterilize all my materials. I then smear E.C, BS, & SA and a mixture of all on 2 slides separated. Then I air-dry the slides but not so much, because it can burn my bacteria. After I put a drop of crystal violet on top of all the section of bacteria on my slides. I proceed to wait a minute for the crystal violet to soak on the bacteria. After the minute is over I make sure the water isn’t running quick enough to take the bacteria off my slides, I then proceed to wash my slides with dripping water. Then I add Gram iodine for a minute and wash it off with the dripping water. After that step is completed I spray alittle of alcohol on my slides where the bacteria sits for 30 seconds ten proceeds to wash it off with water. Then I add Safranine for one minute. After that is completed, I look for the bacteria on the slides.

Results Purple | Gram +ve | | | Bacillus subtilis | | | | | Pink | Gram -ve | | E. coli | Purple | Gram +ve | | Staphylococcusaureus

You May Also Find These Documents Helpful

  • Powerful Essays

    Nt1330 Unit 2 Lab Summary

    • 1237 Words
    • 5 Pages

    What dyes did you used in Gram stain? Crystal violet, gram’s iodine, and safranin violet.…

    • 1237 Words
    • 5 Pages
    Powerful Essays
  • Good Essays

    A: In wet mount stain, color of the slide is same gray, colorless and glossy. Liquid or fluid appearance is visible. Bacteria and background don’t have a much of difference in color. Although, bacteria are defined and visible noted. Most of the time they are single round cells. In direct stain with crystal violet, again background is colorless and glossy. Bacteria is blue and some uneven grouping is noted. In this example the dye methylene blue stain is used. In indirect stain with Congo red stain, background is again colorless and glossy. Bacteria are orange to red and fine defined. At smaller magnified example they are grouping in uneven clusters, but in highest magnifier we can see single bacteria dark cell.…

    • 472 Words
    • 2 Pages
    Good Essays
  • Powerful Essays

    Double Unknown Lab Report

    • 1229 Words
    • 5 Pages

    The following tables are each corresponding to either the Gram-positive sample or Gram-negative sample and include the tests utilized along with their results all in the order each test was performed. In section 10, Figures, there are some pictures corresponding to the stains performed. In Figure 1, the Gram-positive stain, you can clearly see the purple color and cocci morphology in grapelike clusters. Figure 2 is the Gram-negative stain with the safranin color and shows bacilli cellular morphology.…

    • 1229 Words
    • 5 Pages
    Powerful Essays
  • Good Essays

    bio 260

    • 428 Words
    • 2 Pages

    What is the role of the alcohol wash in the Gram Stain? What color would all bacteria become at the end of the staining procedure if the alcohol step in the gram stain was omitted?…

    • 428 Words
    • 2 Pages
    Good Essays
  • Good Essays

    Gram staining is a technique that was invented by Hans Christian Gram in 1882 (Cantey & Doern, 2015). First, a drop of water was placed on a microscope slide. Using a loop and aseptic techniques (working by an open flame to prevent contamination, and sterilizing the loop in the flame until red hot), a small amount of bacteria from the master plate was mixed with the water then streaked onto the slide. The slide was allowed to dry, then the bacteria was heat fixed onto the slide by running it through the flame twice. A clothespin was attached to the slide and the slide was placed on a staining tray. The smear was covered with crystal violet and allowed to stain the bacteria for two minutes. Then, the slide was rinsed with distilled water at an angle, not directly onto the smear. Grams iodine was added to the slide and allowed to sit for one minute and rinsed again with distilled water. Next, the differentiation step occurred (Bartholomew & Mittwer, 1952) using a small amount of alcohol (about 8 drops) rinsed over the slide, followed immediately by rinsing with distilled water. Safranin was then added to the slide and allowed to sit for 30 seconds then rinsed with distilled water. After blotting the slide with bibulous paper, the slide was viewed under the microscope at 1000X magnification. The counterstain Safranin can be substituted for another…

    • 1550 Words
    • 7 Pages
    Good Essays
  • Satisfactory Essays

    Lab 3 Questions

    • 299 Words
    • 2 Pages

    Why is it important to heat fix the bacteria prior to the staining procedure? This procedure is done to kill any bacteria that may be on the slide already and this will insure a clean slide.…

    • 299 Words
    • 2 Pages
    Satisfactory Essays
  • Better Essays

    Micro lab quiz 2

    • 1967 Words
    • 8 Pages

    The Gram stain separates bacteria into two major groups: Gram positives that stain purple, and Gram negatives that stain pink or red.…

    • 1967 Words
    • 8 Pages
    Better Essays
  • Good Essays

    MicroOrganism

    • 777 Words
    • 4 Pages

    3.) Place 3-5 drops, or enough to cover the bacteria, of crystal violet stain onto the slide and let…

    • 777 Words
    • 4 Pages
    Good Essays
  • Better Essays

    Unknown Project

    • 2236 Words
    • 9 Pages

    This test is performed to get a description of the make-up of the cell membrane of the bacteria. A gram positive bacteria will display a blue/violet pigmentation where a gram negative bacteria will display a pink/red pigmentation. This is due to the outer membrane of gram negative bacteria. My unknown bacteria displays a pink color hinting the presence of a gram negative bacteria.…

    • 2236 Words
    • 9 Pages
    Better Essays
  • Good Essays

    Gram Staining Lab

    • 423 Words
    • 2 Pages

    Prokaryotes are a large group of organisms with no membrane bound organelles. They consist of two domains: Archaea and Bacteria. These organisms are only found in extreme environments such as volcanoes. Prokaryotes are still being researched and are a very diverse group. In this lab we focused on trying to identify if the bacteria found had a lot of peptidoglycan by gram staining. Testing this could be done by using a Petri dish full of agar and testing different bacteria on it to see if the bacteria obtained is gram positive or gram negative. My hypothesis is there will be a lot of bacterial growth on all of the plate.…

    • 423 Words
    • 2 Pages
    Good Essays
  • Good Essays

    Unknown Lab Report

    • 1149 Words
    • 5 Pages

    Prepare two slides for gram staining and viewing under a microscope. {Either my gram-stain slides were bad or the microscopes I chose for viewing were not good. No bacteria were found under the microscope.}…

    • 1149 Words
    • 5 Pages
    Good Essays
  • Good Essays

    One possible source of contamination may be the produce that is grown and distributed locally at a farmer’s market. A test sample of some of the produce revealed evidence of bacterial contamination. The bacteria sample was tested in a microbiology lab and showed the results that follow. The laboratory also performed a Gram stain of the isolated bacteria and ran a number of biochemical tests to aid identification. The biochemical tests were assayed using the Analytical Profile Index (API) 20E system for identification of Enterobacteriaceae and other gram-negative…

    • 891 Words
    • 4 Pages
    Good Essays
  • Satisfactory Essays

    Shigella Flexneri

    • 494 Words
    • 2 Pages

    | Glucose fermented to acid and protein in media was broken down to alkaline end product…

    • 494 Words
    • 2 Pages
    Satisfactory Essays
  • Good Essays

    Unknown Bacteria Essay

    • 614 Words
    • 3 Pages

    Secondly both bacteria had to be gram stained, so that they could be identified as gram-positive or as gram-negative. The same gram staining method was used for both bacteria, which consisted of inoculating each bacteria into a…

    • 614 Words
    • 3 Pages
    Good Essays
  • Good Essays

    As I recall from earlier that semester, the first test that needed to be performed was the Gram stain. The Gram stain is a staining technique that aids in distinguishing cell wall characteristics and the cellular morphology. Bacteria will stain Gram positive, which is a purple color, or Gram negative, which is a pink color. Also, the Gram stain can help to identify the shape of the bacterium. The three basic shapes are cocci, bacilli and spirilli, which means that cocci are berry shaped, bacilli are rod shaped and spirilli are spiral shaped. My unknown bacteria stained purple which means that it is Gram-positive and was berry shaped meaning that the bacterium is cocci. These results have led me to the next step for me to master, the catalase test.…

    • 749 Words
    • 3 Pages
    Good Essays