Graph 1 Lab Report
In Graph 1, each line of color represents a different level of pH added to the solution. When pH 9 was added, it produced the highest rate of reaction (the most oxygen was produced), whereas when the more acidic pH 5 was added, the rate of reaction was much slower, and less efficient. The higher the oxygen evolved (gas produced), the more reactions were being produced/higher rate of reaction. Different types of enzymes’ reaction proportions differ based on the pH being added. For example, intestinal enzymes may react differently to pH than liver enzymes (catalase) which was tested.
The hypothesis was proved to be correct due the rate of reaction increasing as the pH levels got more basic. Based on Graph 1, the slope of the pH 9 line was more
steep compared to the other two. This shows that not only did more reaction occur, but also at a faster rate. This conveys that less denaturing occurred as the reaction went at a moderate pace, rather than slowing down immediately, rendering the enzyme dysfunctional, as the lower pH 5, was beginning to do. Based on the results, we can conclude that the pH’s 7 to 9 are optimum for the liver enzyme.
After researching the topic, information indicates that liver enzymes tend to work best with pH 7. Table and Graph 1 suggest that pH 9 was the optimum level for fastest rate of reaction. Some errors that could have occurred could be because of inaccurate amounts of catalase in the beaker. Human error could be another aspect that affected the experiment in terms of correct results, such as not holding the beaker steadily to provide accurate information on the oxygen evolved, or incorrect readings of data. If there was a holder to keep the water and beaker with hydrogen peroxide and catalase more steady, it would provide more reliable results.
If the theories of the lab were further explored, possibly, the following tests could be used for a deeper understanding of the major topics relating to pH.
Does pH have a direct affect on the denaturing of proteins in other substances?
How do pH and Temperature levels correlate regarding rate of reaction in enzymes?
The hypothesis was proved to be correct due the rate of reaction increasing as the pH levels got more basic. Based on Graph 1, the slope of the pH 9 line was more
steep compared to the other two. This shows that not only did more reaction occur, but also at a faster rate. This conveys that less denaturing occurred as the reaction went at a moderate pace, rather than slowing down immediately, rendering the enzyme dysfunctional, as the lower pH 5, was beginning to do. Based on the results, we can conclude that the pH’s 7 to 9 are optimum for the liver enzyme.
After researching the topic, information indicates that liver enzymes tend to work best with pH 7. Table and Graph 1 suggest that pH 9 was the optimum level for fastest rate of reaction. Some errors that could have occurred could be because of inaccurate amounts of catalase in the beaker. Human error could be another aspect that affected the experiment in terms of correct results, such as not holding the beaker steadily to provide accurate information on the oxygen evolved, or incorrect readings of data. If there was a holder to keep the water and beaker with hydrogen peroxide and catalase more steady, it would provide more reliable results.
If the theories of the lab were further explored, possibly, the following tests could be used for a deeper understanding of the major topics relating to pH.
Does pH have a direct affect on the denaturing of proteins in other substances?
How do pH and Temperature levels correlate regarding rate of reaction in enzymes?