In 1577, the Franciscan missionaries arrived in Manila, and in 1578 they started evangelizing Laguna, Morong (now Rizal), Tayabas (now Quezon) and the Bicol Peninsula. Fathers Juan de Placencia and Diego de Orepesa were the earliest Franciscans sent to these places. From 1580, the towns of Bay, Caliraya, Majayjay, Nagcarlan, Liliw, Pila, Santa Cruz, Lumban, Pangil and Siniloan were founded. In 1678, Fray Hernando Cabrera founded San Pablo de los Montes (now San Pablo City) and built a wooden church and convent considered as the best and finest in the province.
In 1670, delimitation of borders were made between Lucban, Majayjay and Cavite. The populous town at that time was Bay, the capital of the province until 1688, when the seat of the provincial government was moved to Pagsanjan, and later, in 1858, to Santa Cruz. In 1754, the Province of Laguna and Tayabas were divided, with the Malinao River separating the towns of Majayjay and Lucban.
The province became a bloody battle ground for the Chinese during the two instances that they rose in revolt against Spain. In 1603's, the Chinese made their last stand in the mountains of San Pablo, and in 1639, they fortified themselves in the highlands of Cavinti and Lumban, surrendering in Pagsanjan a year later.
The loyalty of the people of Laguna to the crown was tested during the British invasion (1762-1764) when thousands of Filipinos rallied to its defense. When a detachment of British troops under