The object of this particular experiment is to gain knowledge on diffusion across a plasma membrane, which is selectively permeable. Diffusion is the movement of a substance from a region of high concentration to lower concentration. Experiment 2 was performed to see how the substance’s, starch, and diffusion process across a plasma membrane is affected by amylase. We prepared a simulated digestive tract of a caterpillar, as the plasma membrane, to see if the amylase breaking down the cooked starch affected the diffusion process. My hypothesis for this experiment is that the amylase breaking down the starch will allow the glucose to diffuse out of the plasma membrane easier than if amylase wasn’t present with the starch. The null hypothesis for this experiment is that when amylase is added with starch it does not make …show more content…
We then securely tied both ends of the tubing and placed it in a large beaker, two-thirds filled with water. We then added 4 pipettes of Lugol’s reagent to the beaker and let it sit for one hour. Next, we took a control with just starch in dialysis tubing and placed it in a large beaker, two-thirds filled with water. We added 4 pipettes of Lugol’s to the water and also let this sit for 1 hour. At the beginning of the experiment, the starch was a cream color, the amylase was clear, the mixture of amylase and starch was a murky cream color and the large beaker filled 2/3 with water with 4 pipettes of Lugol’s was a yellow, brown color. At the end of 1 hour, the large beaker still had a yellow brown color, however the starch in the dialysis tubing of the control had turned a bluish brown color. The glucose level in the dialysis tubing with the mixture of amylase and starch was 200 and the glucose level in the large beaker filled 2/3 with water and 4 pipettes of Lugol’s was