How Does Dr. Antonini's Report

My results are consistent with those, conducted by Dr. Antonini who used the same type of welding fume particles (MMAW: manual metal arc welding). However, welding fume doses at 10 mg/kg and 20 mg/kg in my experiment were twice and 4 times, respectively, the dose that was used in Dr. Antonini’s paper [6]. In blood, Dr. Antonini found a 1.5-fold increase in Mn levels, whereas in my experiment I found an 8-fold increase in blood Mn after the 20 mg/kg dose. Both results are consistent since the 20 mg/kg dose in my experiment was 4 times more than the dose in Dr. Antonini’s paper. In the striatum, Dr. Antonini found an elevation in Mn levels by 1.7 times, whereas I found an increase in Mn levels by 2.5-3.5 times. This larger increase is due to increasing the dose from 10 mg/kg to 20 mg/kg which are 2-4 times more than the dose used in Antonini’s paper. These results suggest a dose-dependent increase in Mn accumulation in the striatum after pulmonary exposure to WF particles. Since 10 mg/kg dose shows trending difference in Mn accumulation in the striatum, I am interested to continue the exposure with 10 mg/kg.
They found a slow increase in blood Mn levels from 7 ppb to 12 ppb from day 1 to day 5. However, in my experiment there was a slower increase from 15.8 ppb to 16.3 ppb although the dose that I used in Figure 5 was five times the dose in the rat study. This result suggests even a slower absorption of WF particles compared with MnO2 particles. Therefore, multiple dose kinetics with blood sampling every week for 4-6 weeks will elucidate the effect of Hfe deficiency on Mn absorption after pulmonary exposure to WF