Introduction- diffusion is a passive transport where molecules move from areas of higher concentration to areas of low concentrations. Although the molecule 's movement is random, equilibrium is being sought out. Osmosis is a type of diffusion only It involves water. In osmosis water diffuses from the area of high concentration to an area of low concentration, to diffuse the more molecule rich side. Because cells have selectively permeable membranes, meaning certain substances can flow through the membrane, this allows osmosis to happen to cells in certain solutions. Osmosis can affect the cell in three different types of ways. The cell can become hypertonic,meaning the concentration …show more content…
outside the cell is higher than the concentration inside the cell and this is where the cell will shrink as the water diffuses out the cell to dilute the higher outside concentration. The cell can also become hypotonic, this is where the concentration of the solute outside the cell is lower than inside the cell and the water will diffuse into the cell to dilute the higher inside concentration, this causes the cell to grow as it fills. Finally the cell can also become isotonic where the molarity of the solute is equal outside and inside the cell.
This lab was used to investigate osmosis by observing how the different solutions of sugar water will affect the mass of pieces of potato. By making three cups with the same amount of water, but with different molarity 's of sugar and placing three, almost equivalent, pieces of potato in each cup for two days, the affect of osmosis was shown through how the water diffused in or out of the potato to enlarge and shrink the potatoes at the end of the two days.
Hypothesis- if the molarity of sugar in a solute is greater than in a piece of potato, then the potato will lose mass because of osmosis
Materials- Potato. Sugar. Cups. Water. Scale. Parafilm.
Procedure-
1.) Gather all materials.
2.) Using the spore, pop out three pieces of potato (weigh each of them).
3.) Take the cups and label one "no sugar", label one "medium sugar" and pour 8.03g of sugar into it, then label the final cup "lots of sugar" and pour 30.12g of sugar in it.
4.) Poor 50 mL of water into each cup.
5.) Put one piece of potato in each cup (remember to record the mass of the potato that was in each cup).
6.) Let solutions sit for two days.
7.) Take potatoes out of solutions.
8.) Weigh each on a scale separately.
9.) Record results.
Data- No Sugar. Medium Sugar. Lots of Sugar. Amount of sugar: 8.03g. 30.12g
Mass of potato before: 5.57g. 5.35g. 4.88g
Mass of potato after: 6.82g. 4.40g. 2.94g.
Mass changed by: +1.25g. -1.13g. -1.94g
Results- The solution had a Higher concentration the potato lost more mass then when the solution had medium concentration or no sugar concentration. The solution with the most sugar concentration lost 1.94 g of mass while the solution with medium concentration lost 1.13 g and the solution will with no sugar added tends gained 1.25 g of
mass.
Analysis- the potato in solution of high molarity lost the most mass put of the three other potatoes. Due to osmosis, the potato piece became hypertonic as the water left the potato in trying to further dilute the solution outside of it. Because the water diffuse out of the potato, it lost 1.94 g of mass. This also goes for the potato in the medium concentrated solution. This potato lost 1.13 g of mass as the water left it due to osmosis because the water in the potato diffused out to try and dilute the concentrated solution outside of it. This potato wasn 't as quite hypertonic as the heavily concentrated solution, but it still lost a fair amount of mass. As for the potato in the water with no sugar, it gained 1.25 g of mass from the occurrence of osmosis. Here the potato grew in mass because inside the potato was more concentrated then the water outside of it, osmosis cause the water to diffuse into the potato, making the potato become hypotonic. To better this lab I thinks different variable for a cell membrane might be more useful and produce less possible error. Because there was no way to measure how concentrated the potato was inside, we had to inquire wheatear it have more or less molarity then its solution. Another erroneous factor might be the amount of sugar and water use. To improve for a future lab, maybe the cup with the most molarity should be fully saturated with sugar and the medium concentrated solution should be measured out to be half of the possible saturation that 50ml of water can hold. This could improve the lab to see more organized and accurate results.
Conclusion- Our hypothesis that if the molarity of sugar in the solute is greater then in a piece of potato, then the potato will lose mass because of osmosis, was supported by the results. Our hypothesis was supported because as we guess the solution with the highest molarity lost the most mass because of osmosis and because the water from inside the potato diffused out and into the solution surrounding it. This shows that the more sugar concentration there was in a solution the more mass a potato core will lose. I assume other groups got the same results because the factors of osmosis. Other than the mass, I noticed the potato that became hypotonic was more slimy as it came out of the water, and I think this could be due to the fact that more water was being held inside the potato.
Acknowledgments- I acknowledge my lab partners for doing an equal share of the work and helping the lab be carried out as directed. I would also like to acknowledge my sister, Maggie, for doing homework with me every night and keeping me focused enough to finish this report. Finally I will also acknowledge the whom ever created the iPad because it was helpful in tracking data for this lab and taking notes as well as typing the report.
References-
Nowicki, Stephen. Biology. [Austin, Texas]: Holt McDougal, 2010. Print. "Osmosis." Merriam-Webster. Merriam-Webster, n.d. Web. 23 Sept. 2013. "Miller and Levine Biology ©2010: A Biology Curriculum by Pearson." Pearsonschool.com:. N.p., n.d. Web. 23 Sept. 2013.