Hypothesis Chromatography Lab Report
Problem
How can you tell pigment separation by using Chromatography?
Objective
To prepare a chromatogram, separate pigments in a leaf and interpret the chromatogram.
Hypothesis
If I am to put a chromatography paper into a solvent, then it would separate the pigments depending on their Rf value. I think the pigments will separate in this order: Chlorophyll A, Chlorophyll B, Carotene, and Xanthophyll.
Introduction Chlorophyll is the molecule that absorbs sunlight and uses its energy to synthesize carbohydrates from CO2 and water. This process is known as photosynthesis and is the basis for sustaining the life processes of all plants. Since animals and humans obtain their food supply by eating plants, photosynthesis can be said to be the source of our life also. As the chlorophyll in leaves decays in the autumn, the green color fades and is replaced by the oranges and reds of carotenoids. Xanthophyll is the yellow pigment in plants that, like chlorophyll, is responsible for the production of carbohydrates by photosynthesis. Chromatography is any of various techniques for the separation of complex mixtures that rely on the differential affinities of substances for a gas …show more content…
Also the strip was bent at the top so there could have been a slight error in measuring the migration of the solvent. My hypothesis was somewhat correct. I was correct that if I am to put a chromatography paper into a solvent, then it would separate the pigments depending on their Rf value. But I was wrong when I said the order. The order was Chlorophyll B, Chlorophyll A, Xanthophyll, and Carotene. Some improvement that could have been made was that the paper strips were previously cut to their specific lengths. Also the timing could have been more accurate with the
How can you tell pigment separation by using Chromatography?
Objective
To prepare a chromatogram, separate pigments in a leaf and interpret the chromatogram.
Hypothesis
If I am to put a chromatography paper into a solvent, then it would separate the pigments depending on their Rf value. I think the pigments will separate in this order: Chlorophyll A, Chlorophyll B, Carotene, and Xanthophyll.
Introduction Chlorophyll is the molecule that absorbs sunlight and uses its energy to synthesize carbohydrates from CO2 and water. This process is known as photosynthesis and is the basis for sustaining the life processes of all plants. Since animals and humans obtain their food supply by eating plants, photosynthesis can be said to be the source of our life also. As the chlorophyll in leaves decays in the autumn, the green color fades and is replaced by the oranges and reds of carotenoids. Xanthophyll is the yellow pigment in plants that, like chlorophyll, is responsible for the production of carbohydrates by photosynthesis. Chromatography is any of various techniques for the separation of complex mixtures that rely on the differential affinities of substances for a gas …show more content…
Also the strip was bent at the top so there could have been a slight error in measuring the migration of the solvent. My hypothesis was somewhat correct. I was correct that if I am to put a chromatography paper into a solvent, then it would separate the pigments depending on their Rf value. But I was wrong when I said the order. The order was Chlorophyll B, Chlorophyll A, Xanthophyll, and Carotene. Some improvement that could have been made was that the paper strips were previously cut to their specific lengths. Also the timing could have been more accurate with the