5.05 Ink Chromatography
5.05 Ink Chromatography
Basic Lab Components
Background Information and Research:
1. Paper chromatography type of method that is used to separate mixtures of substances from a solution.
2. There are many uses for paper chromatography, especially concerning the fields of chemistry and biology. One use is to allow scientists to detect any sort of contaminants is rainwater. Another use is to identify clues at a crime scene such as blood, ink, or drugs. However, the more common use is to make sure food company’s processes are working correctly and that they are producing the correct product.
Research Sites:
http://jh399.k12.sd.us/Lab%20Activity/Chemistry/ch22chromatography.pdf http://www.discoveriesinmedicine.com/Bar-Cod/Chromatography.html#b http://www.chemguide.co.uk/analysis/chromatography/paper.html
Purpose
The purpose of this laboratory investigation is to observe how chromatography can be used to separate mixtures of a solution.
Materials
Four different markers, one being black
Rubbing alcohol or isopropyl alcohol
2 Coffee filters
2 Tall glasses or plastic cups
Pencil
Ruler
Tape
Table salt
Water
Measuring cups/spoons
Clean pitcher or 2-liter bottle
Procedure
Step 1. Take a coffee filter and cut it into a rectangle with the measurements of three cm by nine cm. Repeat once more, as two coffee filters in the shape of a rectangle will be needed.
Step 2. Using a pencil, not a pen, draw a line one cm from the edge of one of the coffee filters.
Step 3. With a pencil, make four dots along the line just drawn about 0.5 cm apart.
Step 4. Use four different colored markers to draw a dot on each of the pencil marks on the coffee filter strip. Wait until the ink is dry, and then add more color to each of the dots. Hints: 1. One of the colored markers should be black. 2. Restrain from using permanent markers.
Step 5. Make the salt solution by combining 1/8 teaspoon of salt and three cups of
Basic Lab Components
Background Information and Research:
1. Paper chromatography type of method that is used to separate mixtures of substances from a solution.
2. There are many uses for paper chromatography, especially concerning the fields of chemistry and biology. One use is to allow scientists to detect any sort of contaminants is rainwater. Another use is to identify clues at a crime scene such as blood, ink, or drugs. However, the more common use is to make sure food company’s processes are working correctly and that they are producing the correct product.
Research Sites:
http://jh399.k12.sd.us/Lab%20Activity/Chemistry/ch22chromatography.pdf http://www.discoveriesinmedicine.com/Bar-Cod/Chromatography.html#b http://www.chemguide.co.uk/analysis/chromatography/paper.html
Purpose
The purpose of this laboratory investigation is to observe how chromatography can be used to separate mixtures of a solution.
Materials
Four different markers, one being black
Rubbing alcohol or isopropyl alcohol
2 Coffee filters
2 Tall glasses or plastic cups
Pencil
Ruler
Tape
Table salt
Water
Measuring cups/spoons
Clean pitcher or 2-liter bottle
Procedure
Step 1. Take a coffee filter and cut it into a rectangle with the measurements of three cm by nine cm. Repeat once more, as two coffee filters in the shape of a rectangle will be needed.
Step 2. Using a pencil, not a pen, draw a line one cm from the edge of one of the coffee filters.
Step 3. With a pencil, make four dots along the line just drawn about 0.5 cm apart.
Step 4. Use four different colored markers to draw a dot on each of the pencil marks on the coffee filter strip. Wait until the ink is dry, and then add more color to each of the dots. Hints: 1. One of the colored markers should be black. 2. Restrain from using permanent markers.
Step 5. Make the salt solution by combining 1/8 teaspoon of salt and three cups of