Immobile Cells Lab Report

Discussion/Conclusion: It was hypothesized that the absence of calcium, or the presence of the following inhibitors: colchicine, and neomycin; will prevent flagella regeneration in Chlamydomonas reinhardtii, following the deflagellar treatment of a pH shock and neutralization, by depicting a constant percentage of immobile cells. Prior to the lab series, it was expected that the positive control would present a decreasing percentage of immobile cells, and the negative control would present an increasing percentage of immobile cells. The experimental groups, testing the hypothesis, was inferred to show a constant number of immobile cells, after the 60-minute light treatment. Colchicine is a substance that reduces inflammation, and is typically characterized for the inhibition of tubulin (Colchicine). In the first experiment, the data expressed that the positive control and experimental group of Group 4b expressed a decreasing percentage of immotile cells over 60-minutes at 19%, and 57%, respectively. Additionally, the negative control increased the percentage of immotile cells over 60-minutes at 114%. The data for the experimental
One reason was due to the relative distance of the light source to the sample. It was discovered, after the third experiment, that the samples were heated. The increase in temperature was harmful to the cells and may have killed some cells. The dead cells were counted as immobile during the census. Another reason, was the longevity of the acid shock. The acid shock of 60 seconds may have been life threatening for some of the cells and the dead cells were counted as immobile during the census. This experiment may be done differently by ensuring that the relative distance of the lamp was at a predetermined safe distance away from the cells and that the length of time the cells were in the acid shock was, also, at a predetermined safe amount of