cell bio lab report

Cell Surface Glycoprotein Receptor Analysis Using Concanavalin A

Purpose:
During this experiment we compared the hemagglutination reaction of control Con A solution at 2 mg/ml in Con A buffer with the hemagglutination reaction of your own purified Con A sample that you diluted previously at 2 mg/ml in Con A buffer. The purpose of this lab was to determine the strength of the reaction by performing serial dilutions on both the Con A sample and the control Con A sample, and determine through observations whether or not addition of galactose or mannose will inhibit this reaction. I hypothesize that the Con A + galactose solutions will have partial agglutination and partial no agglutination, and the Con A + mannose solutions will have all no agglutination.
Results:
Rows A and B had half agglutination and half no agglutination, while row C had all no agglutination. Row D had half agglutination and half partial agglutination, while row E had 4 columns with agglutination and 8 columns with no agglutination. Row F had complete agglutination throughout.
Con A reaction plate (Row/Column)
Reaction
A1-A6 (Control)
Agglutination/inhibited
A7-A12 (Control)
No Agglutination/not inhibited
B1-B6 (Con A + galactose)
Agglutination/inhibited
B7-B12 (Con A + galactose)
No Agglutination/not inhibited
C1-C12 (Con A + mannose)
No Agglutination/not inhibited
D1-D5 (Sample)
Agglutination/inhibited
D6-D12 (Sample)
Partial agglutination/inhibited
E1-E4 (Con A + galactose)
Agglutination/inhibited
E5-E12 (Con A + galactose)
No Agglutination/not inhibited
F1-F12 (Con A + mannose)
No agglutination/not inhibited
G1-G12 ((-)Control)
Partial agglutination/inhibited
H1-H12 (RBCs)
Partial agglutination/inhibited

Discussion:
My hypothesis was proven correct, the Con A + galactose solutions did have partial agglutination and partial no agglutination, and the Con A + mannose solutions did have all no agglutination. This means that in the Con A + galactose