Cell Biology Lab Manual
Mammalian Cell Culture Lab Manual
Student guide of Cell Biology Laboratory Techniques
2012
Jay M. Feldman
Cell Biology Professor Lagier
11/26/2012
Mammalian Cell Culture Lab Manual
Student guide of Cell Biology Laboratory Techniques
2012
Jay M. Feldman
Cell Biology Professor Lagier
11/26/2012
Table of Contents
I. Introduction * About the author page 2 * Preface page 3
II. Laboratory Techniques * Cell Culture Technique page 4-5 * Sterile Technique page 6-7 * Media Preparation page 8-9 * Seed cells page 10 * Subculture page 11-12 * Cell count page 13-14 * Cryopreservation page 15
III. Laboratory Experiments * Intentional Contamination page 16-17 * Tonicity page 18-19 * DNA Transfection page 20-23 * Cytoskeleton page 24-25
IV. * Answer Key page 26-27 * Works Cited page 28
About the Author
Hi there! My name is Jay Feldman and I am a junior at Florida Gulf Coast University. I am biology major with a concentration in pre-professional studies. As a 20 year-old full time student, I also work part-time in the emergency room at NCH North Collier. My job title is an emergency department scribe and I have held this position for one year. In my free time I enjoy weight lifting, basketball, and partying with my friends. I am a proud member of Sigma Phi Epsilon fraternity as well as the beach volleyball club and the men’s indoor volleyball team at FGCU. My goal in life is to become a big time plastic surgeon in the city, I love medicine and I work hard every day to achieve my goals. I hope you enjoy my lab manual, be nerdy!
Preface
This laboratory manual was created in order to direct future students through to various laboratory procedures and concepts that a student will learn throughout a semester of cell biology. Following the guidelines in this lab manual will direct you in the techniques to carry out procedures, keep track of
Student guide of Cell Biology Laboratory Techniques
2012
Jay M. Feldman
Cell Biology Professor Lagier
11/26/2012
Mammalian Cell Culture Lab Manual
Student guide of Cell Biology Laboratory Techniques
2012
Jay M. Feldman
Cell Biology Professor Lagier
11/26/2012
Table of Contents
I. Introduction * About the author page 2 * Preface page 3
II. Laboratory Techniques * Cell Culture Technique page 4-5 * Sterile Technique page 6-7 * Media Preparation page 8-9 * Seed cells page 10 * Subculture page 11-12 * Cell count page 13-14 * Cryopreservation page 15
III. Laboratory Experiments * Intentional Contamination page 16-17 * Tonicity page 18-19 * DNA Transfection page 20-23 * Cytoskeleton page 24-25
IV. * Answer Key page 26-27 * Works Cited page 28
About the Author
Hi there! My name is Jay Feldman and I am a junior at Florida Gulf Coast University. I am biology major with a concentration in pre-professional studies. As a 20 year-old full time student, I also work part-time in the emergency room at NCH North Collier. My job title is an emergency department scribe and I have held this position for one year. In my free time I enjoy weight lifting, basketball, and partying with my friends. I am a proud member of Sigma Phi Epsilon fraternity as well as the beach volleyball club and the men’s indoor volleyball team at FGCU. My goal in life is to become a big time plastic surgeon in the city, I love medicine and I work hard every day to achieve my goals. I hope you enjoy my lab manual, be nerdy!
Preface
This laboratory manual was created in order to direct future students through to various laboratory procedures and concepts that a student will learn throughout a semester of cell biology. Following the guidelines in this lab manual will direct you in the techniques to carry out procedures, keep track of
Cited: Accessed on 11-1-2012, located on page 6 http://www.ehow.com/facts_5615759_personal-hygiene.html Accessed on 11-3-2012, located on page 15 http://www.mymultiplesclerosis.co.uk/stranger-than-fiction/cryogenics.html