Investigating the Oral Glucose Tolearnce Test
Investigating the oral glucose tolerance test
Aim: To carry out glucose tests on stimulated blood plasma samples if glucose is present in blood plasma.
Risk Assessment:
Hazardous chemical or microorganism being used or made, or hazardous procedure or equipment. Nature of the hazard(s) Source(s) of information Control measures to reduce the risks
Sulphuric acid - Irritant
- Very corrosive CLEAPS Wear eye protection & wear lab coat. Try not to spill chemical.
Potassium manganate - Harmful over 1 ml
- Danger to environment
- Oxidising agent CLEAPS Wear lab coat & wear eye protection. Wash hands thoroughly at the end of the experiment.
Glassware
(glass stirring rod, boiling tubes) - Can break if dropped and could cause bleeding or other damage to skin. Experience in situation Try not to drop glassware and wear eye protection. Keep working area clear at all times.
Method:
1) Measure 10cm3 of 4 mol dm-3 glucose solution into the first boiling tube
2) Measure 5cm3 of 1 mol dm-3 sulphuric acid and add to the boiling tube from 1
3) Reset the stop clock
4) Use a dropping pipette to take 2cm3 potassium manganate VII solution from a measuring cylinder
5) Add the potassium manganate to the boiling tube and starting the stop clock at the same time
6) Stir the contents of the boiling tube with a stirring rod and stop the clock as the solution becomes colourless
7) Record the time taken to go colourless in a results table
8) Wash boiling tube and dropping pipettes thoroughly
9) Repeat steps 2-8 for the remaining glucose solutions.
Glucose concentration
(gdm-3) Time to decolourise
(secs) Mean time
(secs)
2 242 242
4 112, 124 118
6 91 91
8 70,80 75
10 128, 72 100
12 73, 60 66.5
Results Table:
(Graph of results on next page)
Unknown samples:
Time for glucose Time (secs)
A0 300
A0.5 112, 105
A1 98
A2 281
Time for glucose Time (secs)
B0 105
B0.5 74
B1 67
B2 82
Interpretation of Results:
From the graph of my results I can
Aim: To carry out glucose tests on stimulated blood plasma samples if glucose is present in blood plasma.
Risk Assessment:
Hazardous chemical or microorganism being used or made, or hazardous procedure or equipment. Nature of the hazard(s) Source(s) of information Control measures to reduce the risks
Sulphuric acid - Irritant
- Very corrosive CLEAPS Wear eye protection & wear lab coat. Try not to spill chemical.
Potassium manganate - Harmful over 1 ml
- Danger to environment
- Oxidising agent CLEAPS Wear lab coat & wear eye protection. Wash hands thoroughly at the end of the experiment.
Glassware
(glass stirring rod, boiling tubes) - Can break if dropped and could cause bleeding or other damage to skin. Experience in situation Try not to drop glassware and wear eye protection. Keep working area clear at all times.
Method:
1) Measure 10cm3 of 4 mol dm-3 glucose solution into the first boiling tube
2) Measure 5cm3 of 1 mol dm-3 sulphuric acid and add to the boiling tube from 1
3) Reset the stop clock
4) Use a dropping pipette to take 2cm3 potassium manganate VII solution from a measuring cylinder
5) Add the potassium manganate to the boiling tube and starting the stop clock at the same time
6) Stir the contents of the boiling tube with a stirring rod and stop the clock as the solution becomes colourless
7) Record the time taken to go colourless in a results table
8) Wash boiling tube and dropping pipettes thoroughly
9) Repeat steps 2-8 for the remaining glucose solutions.
Glucose concentration
(gdm-3) Time to decolourise
(secs) Mean time
(secs)
2 242 242
4 112, 124 118
6 91 91
8 70,80 75
10 128, 72 100
12 73, 60 66.5
Results Table:
(Graph of results on next page)
Unknown samples:
Time for glucose Time (secs)
A0 300
A0.5 112, 105
A1 98
A2 281
Time for glucose Time (secs)
B0 105
B0.5 74
B1 67
B2 82
Interpretation of Results:
From the graph of my results I can