Ion Exchange Zeolite Case Study
Evaluation of the effects of Ag exchanged zeolite nanocomposite on excisional wound healing in rats.
Introduction
Skin is often injured by wounding or physical trauma. Damages to skin initiate a series of complicated and well-orchestrated events of repair processes ending with complete reestablishment of the penetrate deep in the skin remain a major clinical problem because of morbidity associated with prolonged periods required for repair and regeneration of the injured tissue, bleeding, risk for infections and septicemias, keloids and scar formation 1. Although new therapeutic approaches for such a large and full thickness skin defect treatment have made progress, there is still need for better methods to enhance wound healing and recovery especially in severely wounded patients. One of the approaches for treating wounds is the use of biocompatible composites incorporated with antibacterial agent to improve wound healing and diminish the wound infection risk 2. Ion-exchange zeolite has gained attention due to its renewable nature, good biocompatibility and excellent …show more content…
MCF-7 cells (9×103 cell/well) were incubated in 96-well plates each containing 200 ml of supplemented cell culture media for 24 h at 37°C and 5% Co2. The cells were divided in 4 groups in quadruplicates: blank and the Ag+-Zeolite/gelatin different concentrations of 0.1, 0.5, and 1 mg/ml were treated. After an incubation period of 24 h, the spent media were removed and the plate wells were washed with Phosphate buffered solution. Briefly, 50 IL of 2 mg/ml MTT and 150 IL culture medium were added to each well. The cells were incubated at 37 °C and 5% Co2 for 4 h. Then the media was discarded and dimethyl sulfoxide and Sorenson buffer was added to each well as solubilizer buffer. Finally, absorbance was read using an ELISA plate reader (BioTeck, Bad Friedrichshall, Germany) at 570 nm
Introduction
Skin is often injured by wounding or physical trauma. Damages to skin initiate a series of complicated and well-orchestrated events of repair processes ending with complete reestablishment of the penetrate deep in the skin remain a major clinical problem because of morbidity associated with prolonged periods required for repair and regeneration of the injured tissue, bleeding, risk for infections and septicemias, keloids and scar formation 1. Although new therapeutic approaches for such a large and full thickness skin defect treatment have made progress, there is still need for better methods to enhance wound healing and recovery especially in severely wounded patients. One of the approaches for treating wounds is the use of biocompatible composites incorporated with antibacterial agent to improve wound healing and diminish the wound infection risk 2. Ion-exchange zeolite has gained attention due to its renewable nature, good biocompatibility and excellent …show more content…
MCF-7 cells (9×103 cell/well) were incubated in 96-well plates each containing 200 ml of supplemented cell culture media for 24 h at 37°C and 5% Co2. The cells were divided in 4 groups in quadruplicates: blank and the Ag+-Zeolite/gelatin different concentrations of 0.1, 0.5, and 1 mg/ml were treated. After an incubation period of 24 h, the spent media were removed and the plate wells were washed with Phosphate buffered solution. Briefly, 50 IL of 2 mg/ml MTT and 150 IL culture medium were added to each well. The cells were incubated at 37 °C and 5% Co2 for 4 h. Then the media was discarded and dimethyl sulfoxide and Sorenson buffer was added to each well as solubilizer buffer. Finally, absorbance was read using an ELISA plate reader (BioTeck, Bad Friedrichshall, Germany) at 570 nm