Lab Assignment
Observing Bacteria and Blood
Abstract
The purpose of this exercise is to gain knowledge on basic skills to correctly operate a microscope by viewing blood and bacteria through different magnifications
Hypothesis
This exercise will help me gain knowledge and the correct way to use a microscope. This will also help me for future observations of microbes.
Methodology
Properly set up microscope and made sure it was functioning properly. All lenses were wipe with lens paper and nine different slides were chosen upon inspection, two of which were fresh. Each slide was carefully observed at each magnification (4x, 10x, 40x). Observations were reported in table and drawings of my findings.
Table 1 Specimens | “e” slide | Penicillium with conidia | Bacteria, bacillus form | yeast | Bacteria, coccus form | Bacteria, spirillium form | Prepared yogurt slide | Fresh blood smear | Fresh yogurt slide – 24 hours old |
Observations Specimen | Magnification | Observations | “e” | 150x | “e” is clear to see | | | Edges are not crisp as rest of the image | Penicillium with conidia | 150x | Long, wavy, skinny strands everywhere | | | Top area has dark spots | | | Image seems to be fuzzy in middle area | | 600x | Shapes are being more clear | | | | | | Cocci shapes more visible |
| 1500xoil immersion | Long tails of circles formed | | | Cocci shapes more prominent | | | Image not as clear as previous magnification | Bacteria, bacillus form | 150X | Skinny, stringlike strands | | | Some pieces seem connected | | | Not able to determine shape | | | Looks more like little pieces of hair | | 600x | Strands look more like rods | | | Connected | | | Still seem more stringlike | | 1500Xoil immersion | Rods are more clear to see | | | Some rods seem to be bigger than others | | | Solid, not transparent | Yeast | 150X | Little cluster of balls | | | Some separated,
Abstract
The purpose of this exercise is to gain knowledge on basic skills to correctly operate a microscope by viewing blood and bacteria through different magnifications
Hypothesis
This exercise will help me gain knowledge and the correct way to use a microscope. This will also help me for future observations of microbes.
Methodology
Properly set up microscope and made sure it was functioning properly. All lenses were wipe with lens paper and nine different slides were chosen upon inspection, two of which were fresh. Each slide was carefully observed at each magnification (4x, 10x, 40x). Observations were reported in table and drawings of my findings.
Table 1 Specimens | “e” slide | Penicillium with conidia | Bacteria, bacillus form | yeast | Bacteria, coccus form | Bacteria, spirillium form | Prepared yogurt slide | Fresh blood smear | Fresh yogurt slide – 24 hours old |
Observations Specimen | Magnification | Observations | “e” | 150x | “e” is clear to see | | | Edges are not crisp as rest of the image | Penicillium with conidia | 150x | Long, wavy, skinny strands everywhere | | | Top area has dark spots | | | Image seems to be fuzzy in middle area | | 600x | Shapes are being more clear | | | | | | Cocci shapes more visible |
| 1500xoil immersion | Long tails of circles formed | | | Cocci shapes more prominent | | | Image not as clear as previous magnification | Bacteria, bacillus form | 150X | Skinny, stringlike strands | | | Some pieces seem connected | | | Not able to determine shape | | | Looks more like little pieces of hair | | 600x | Strands look more like rods | | | Connected | | | Still seem more stringlike | | 1500Xoil immersion | Rods are more clear to see | | | Some rods seem to be bigger than others | | | Solid, not transparent | Yeast | 150X | Little cluster of balls | | | Some separated,