Preview

lab report

Good Essays
Open Document
Open Document
2100 Words
Grammar
Grammar
Plagiarism
Plagiarism
Writing
Writing
Score
Score
lab report
INTRODUCTION There are many reasons for knowing the identity of microorganisms. The reasons range from the knowing the causative agent of a disease to knowing the correct microorganism in order to make antibiotics. This study was done by applying the following methods; OF Glucose, Indole Production, and Malonate Utilization test for the identification of an unknown bacterium. The methods will assist in determining the unknown bacterium found in a 55 year old male that was passing blood and mucous in his stool. The patient had no pain but mild discomfort during bowel movements. The doctor noted inflammation of the lining of the rectum. The doctor removed a tissue sample for examination and referred the patient to a gastroenterologist for a barium xray and colonoscopy. While utilizing the methods stated above, the bacterium found was a facultative anaerobe due to the Gram Negative Rods, fermentation, and the production of indole. The specific Gram Negative Enteric Bacilli was Shigella dysenteriae. There are many reasons for knowing how to control bacterial growth as well as such as antimicrobial drugs, which assist the body’s defenses against pathogens. Microorganisms are capable of causing infection or spoilage. Controlling bacterial growth is important in poultry farm businesses that use equipment to decontaminate processing plant fluids before disposal. During quality control and safety evaluations, bacteria growth that needs to be controlled can be found. While controlling bacterial growth, clusters of Gram Positive Cocci were found. It was found some ways to control bacterial growth were disinfection, sterilization, antisepsis, sanitization, moist and dry heat, filtration, and radiation; just to name a few. The specific method used for control depends on various circumstances.

PROCEDURE First, you want to place your test tubes on the bench; one being an unknown labeled as GrowthLab1 and the other is the medium. Specific biochemical tests will be performed



References: 1. Bauman, Robert W. Microbiology with Diseases by Taxonomy. Third Edition. San Francisco: Benjamin Cummings publishing, 2011 2. Intuitive Systems, Inc. Virtual Unknown Microbiology Internet Edition. Version 4.0.8.2. Intuitive Systems Inc., 1999

You May Also Find These Documents Helpful

  • Better Essays

    Negative Unknown Lab Report

    • 2965 Words
    • 12 Pages

    The Unknown Gram Negative bacterium inoculated in a Tryptic Soy broth medium was randomly selected from a group of other unknowns. In order to identify this unknown the seven different types of biochemical tests will be conducted on this unknown bacterium to identify it out of 6 possible bacteria; Escherichia coli(E. coli), Enterobacter aerogenes (E.aerogenes), Klebsiella pneumoniae (K.pneumoniae), Proteus mirabilis(P.miranilis), Pseudomonas aeruginosa(P.aeruginosa), and Salmonella typhimurium (S.typhimurium). The biochemical tests utilized were; Triple Sugar Iron Agar (TSIA) test, Sulfur Indole Motility (SIM) test, Methyl red test and Voges-Proskauer (MR-VP) test, Citrate test, Urea test,…

    • 2965 Words
    • 12 Pages
    Better Essays
  • Good Essays

    Lab Report

    • 1398 Words
    • 6 Pages

    The homogenates provided were made by homogenizing tissues in a sucrose phosphate buffer in a 1:20 ratio. The protein concentration in bovine cells was measured by diluting the homogenate with a 1:5 ratio; 50 microliters of homogenate and 200 microliters of water. Then 5 known protein concentration samples which were 0.4, 0.8, 1.2, 1.6, 2.0 mg/ml of bovine serum were used to determine absorbance with a spectrophotometer. Two additional samples were made; one was blank and the other was for the specific homogenate sample. Then 3 microliters of bradford assay reagent, which indicates the amount of protein present by color, was added to all samples. The spectrophotometer was zeroed at 595 nm. A standard curve was made with the different absorbencies and concentrations. After the linear equation was formed, the unknown sample concentration was determined using the standard curve equation. A Gel Electrophoresis was used to perform a qualitative analysis. The use of 5 microliters of the homogenate was heated to 80 degrees Celsius. Then the homogenate was transferred to a 2-microliter-protein gel sample buffer. Samples loaded on to the gel was run at 100 v and stained with comassie blue; observations were made next lab. (Clendening 2014)…

    • 1398 Words
    • 6 Pages
    Good Essays
  • Satisfactory Essays

    Lab Report

    • 597 Words
    • 3 Pages

    The purpose of this experiment is to see whether or not temperature plays a role in the percent change during diffusion or osmosis.…

    • 597 Words
    • 3 Pages
    Satisfactory Essays
  • Satisfactory Essays

    Lab report

    • 613 Words
    • 3 Pages

    Observations: The color changing process took longer than we had expected. We observed that after putting the penny in the beaker, the zinc powder surround the penny, which helped it changed its color. We couldn’t complete the experiment so when we took the penny from the beaker, the change in the color wasn’t completed. There were some dark spots and some light parts.…

    • 613 Words
    • 3 Pages
    Satisfactory Essays
  • Good Essays

    lab report

    • 623 Words
    • 3 Pages

    Obtain for dry test tubes and number them 1 through 4. Prepare two dry Pasteur pipets with bulbs attached. Place 9.0 mL of hexane, 2.0 ml of acetone, and 2.0 mL of solution of 70% hexane-30% acetone into separate Erlenmeyer flask. Label and stopper each flask. Place 0.3 mL of a solution containing fluorine and fluorenone into a small test tube. Stopper the test tube. Prepare one 10-cm x 4.3 cm TLC with four marks for spotting. Prepare four micropipets to spot the plates.…

    • 623 Words
    • 3 Pages
    Good Essays
  • Good Essays

    Unknown Lab Report

    • 1149 Words
    • 5 Pages

    To identify an unknown bacterial specimen using basic laboratory technique and biochemical tests. The unknown bacteria will be one of the following: Enterococcus faecalis, Staphylococcus saprophyticus, Escherichia coli, Enterobacter aerogenes, Proteus vulgaris, Salmonella [I assume typhimurium], or Shigella [either flexneri or sonnei, we used both in our lab during the semester].…

    • 1149 Words
    • 5 Pages
    Good Essays
  • Good Essays

    Unknown Microorganism

    • 1335 Words
    • 6 Pages

    There are many types of microorganisms and ways to treat each one. Knowing the differences of each is vital to treat a patient correctly. The purpose of this report is to explain the process and steps used to identify a certain microorganism referred to as the unknown.…

    • 1335 Words
    • 6 Pages
    Good Essays
  • Good Essays

    One possible source of contamination may be the produce that is grown and distributed locally at a farmer’s market. A test sample of some of the produce revealed evidence of bacterial contamination. The bacteria sample was tested in a microbiology lab and showed the results that follow. The laboratory also performed a Gram stain of the isolated bacteria and ran a number of biochemical tests to aid identification. The biochemical tests were assayed using the Analytical Profile Index (API) 20E system for identification of Enterobacteriaceae and other gram-negative…

    • 891 Words
    • 4 Pages
    Good Essays
  • Good Essays

    Lab Report

    • 623 Words
    • 3 Pages

    The number of electrons forming a charge cloud around the nucleus is (pick one of the following) greater than; equal to; smaller than the number of protons in the nucleus of the atom.…

    • 623 Words
    • 3 Pages
    Good Essays
  • Powerful Essays

    Lab Report

    • 1310 Words
    • 6 Pages

    The cold pressor test is done by having the subject submerge their hands in a bucket of cold water to determine cardiovascular changes such as blood pressure and heart rate. For this experiment we wanted to know how long participant can keep their feet in the bucket of ice water before they experience any pain while looking at neutral and positive pictures and also rate the intensity of their pain. For this reason we hypothesized that, if participant submerged their feet in a bucket of water while looking at the positive stimulus, it would take them a long time to experience pain and will keep their feet in the bucket of ice for a long time than when participant are looking the neutral stimuli while they have their feet in the bucket of ice, we also hypothesized that heart rate will increase the longer participant keep their feet in the ice water. The null hypotheses was that, if participant submerge their feet in a bucket of water while looking at positive stimulus, it would take them the same amount of time to experience pain as when they are looking at the neutral stimulus and that heart rate will stay the same throughout the experiment…

    • 1310 Words
    • 6 Pages
    Powerful Essays
  • Good Essays

    Microbiology Unknown

    • 2745 Words
    • 11 Pages

    The objective of this report was to identify an unknown microorganism through several differential media tests. Over the course of a couple weeks, ten tests were performed. First, a gram stain was performed, indicating the bacterium was gram negative. An aerotolerance test determined that the bacterium was a facultative anaerobe. Next, a negative result in the methyl red test indicated that no mixed acid fermentation occurred. The DNase test was performed and yielded a positive result. The SIM test provided two outcomes, that the bacterium did not reduce sulfur nor produce indole from tryptophan. Afterwards, the bacterium was determined to be positive for lysine decarboxylation and citrate. The purple broth and triple sugar iron tests both indicated gas production. The purple broth test was positive for fermentation, and the triple sugar iron test indicated that the bacterium fermented glucose and sucrose. Finally, the bacterium was urease negative.…

    • 2745 Words
    • 11 Pages
    Good Essays
  • Good Essays

    Lab Report

    • 1382 Words
    • 6 Pages

    sunlight as soon as possible. This process is called etiolation. Once the plants reach the…

    • 1382 Words
    • 6 Pages
    Good Essays
  • Powerful Essays

    Lab Report

    • 1597 Words
    • 7 Pages

    The purpose of this experiment is to determine how a beam deflects with load and compare the experimental values to the values calculated from the simple formula and exact formula. We take three different eccentricities 75 mm, 55 mm, 35 mm and gradually apply load to them. Taking readings and making graphs to explain the outcome. This kind of experiment is helpful in construction of railways and grids.…

    • 1597 Words
    • 7 Pages
    Powerful Essays
  • Better Essays

    Lab Report

    • 3643 Words
    • 22 Pages

    The types of bodies that are being used in our experiment was Round conrod bar, Square conrod bar and trapezium plate. The main idea that been applied in this experiment was the theory of Moment of Inertia.…

    • 3643 Words
    • 22 Pages
    Better Essays
  • Satisfactory Essays

    Lab Report

    • 802 Words
    • 4 Pages

    If I paint one hand of nails using a base coat and another hand of nails without a base coat, then the nails with a base coat will have a smaller area of chips.…

    • 802 Words
    • 4 Pages
    Satisfactory Essays