Lab Report 5
Title: Mitosis and amount of time spent in each phase. Introduction: The cycle of a cell roughly doubles it volume of cytoplasm and duplicates its genetic information i.e. replicated DNA. Then it divides the genetic information equally into two daughter nuclei. The cell cycle can be divided into two phases interphase and mitotic phase. The time spent in these phases depends upon the type of cell and its particular developmental or physiological state. Cells comprising rapidly growing tissues, like those in the root tip of a plant spend a comparatively long time in the mitotic phase, whereas those cells comprising slow-growing tissues would spend most of their lives in interphase. Non-dividing cells remain in interphase and never enter the mitotic phase. (Lab Manual 64)
Interphase is the synthesis of biological molecules including DNA and duplicated DNA with associated proteins. These comprise the chromatin that begin to condense toward the end of this phase, but are not yet visible. The nucleoplasm has a uniform grainy appearance. Prophase is when the chromatin condenses into the more distinct elongate strands early in prophase. Also nucleoli and the nuclear membrane begin to disappear. The mitotic spindles take formation at opposing ends of the cell. The chromatin is visible, but only in threads. Metaphase is the most distinctive of all stages of mitosis, because it is characterized by the regular arrangement of chromosomes at a central region called the metaphase plate. Now Anaphase beings as duplicate chromosomes separate, moving to opposite poles of the cell. Telophase is marked by the formation of a nuclear membrane around two daughter nuclei. Also chromosomes begin uncoiling and less distinct. Cytokinesis begins at this stage, which is shown with the development of a cell plate in plants cells. Hypothesis: Prophase will be the phase that the cells spend the most time in, because so many things are occurring as opposed to other cells. Chromatins
Interphase is the synthesis of biological molecules including DNA and duplicated DNA with associated proteins. These comprise the chromatin that begin to condense toward the end of this phase, but are not yet visible. The nucleoplasm has a uniform grainy appearance. Prophase is when the chromatin condenses into the more distinct elongate strands early in prophase. Also nucleoli and the nuclear membrane begin to disappear. The mitotic spindles take formation at opposing ends of the cell. The chromatin is visible, but only in threads. Metaphase is the most distinctive of all stages of mitosis, because it is characterized by the regular arrangement of chromosomes at a central region called the metaphase plate. Now Anaphase beings as duplicate chromosomes separate, moving to opposite poles of the cell. Telophase is marked by the formation of a nuclear membrane around two daughter nuclei. Also chromosomes begin uncoiling and less distinct. Cytokinesis begins at this stage, which is shown with the development of a cell plate in plants cells. Hypothesis: Prophase will be the phase that the cells spend the most time in, because so many things are occurring as opposed to other cells. Chromatins
References: Preston, R.L., & Riddle, W. A. (2011). Laboratory manual for BSC 197 Molecular and Cellular Basis for Life. Normal, IL: Department of Biological Sciences.