Miss.Tatu
Biology
March 5, 2015
Introduction
In the Mitosis lab an onion root was observed under a microscope to observe whether or not cell division takes place. Also, whether cells replicate in order for it to reproduce. By observing the onion root under the microscope it allows many layers to be looked at through each cell division. The goal of Mitosis is the replication of cells to produce multiple like cells. The steps involved in Mitosis are what is known as the PMAT stages, which are the following: Prophase, Metaphase, Anaphase and Telephase. In the first stage Prophase the cell is starting to curl up into a circular ball. The next step Metaphase is when the cell gets completely curled up into a ball. The third stage Anaphase the cell starts to divide and in the final stage Telephase the cells separate. In the Mitosis lab an onion root was observed under a microscope. In observing the cell it was important to zoom in 40x to enlarge the root so each stage of the cell could be identified. In the stages of Mitosis the focus will be on cell division of the onion root and how many cells were found on the onion root.. “ If the number of cells is multiplied, then the cells completed Mitosis.
Materials and Methods microscope -slides onion root cover slip knife In this lab we received one microscope per group. We needed to be sure the microscope was open to the widest setting and the focus was in. The next step was to cut a piece of onion root and place it on the slide and than to place a cover slip over the top of the slide. Next, we had to take a slide and place it on the microscope stage. We needed to use the adjustments on the microscope to zoom in and out to get a clear view of the onion root and bring the onion root into focus to be able to see the cells. Our focus was on the four stages of Mitosis, being Prophase, Metaphase, Anaphase and Telephase. After our observation we recorded the amount of cells in each stage.