The homogenates provided were made by homogenizing tissues in a sucrose phosphate buffer in a 1:20 ratio. The protein concentration in bovine cells was measured by diluting the homogenate with a 1:5 ratio; 50 microliters of homogenate and 200 microliters of water. Then 5 known protein concentration samples which were 0.4, 0.8, 1.2, 1.6, 2.0 mg/ml of bovine serum were used to determine absorbance with a spectrophotometer. Two additional samples were made; one was blank and the other was for the specific homogenate sample. Then 3 microliters of bradford assay reagent, which indicates the amount of protein present by color, was added to all samples. The spectrophotometer was zeroed at 595 nm. A standard curve was made with the different absorbencies and concentrations. After the linear equation was formed, the unknown sample concentration was determined using the standard curve equation. A Gel Electrophoresis was used to perform a qualitative analysis. The use of 5 microliters of the homogenate was heated to 80 degrees Celsius. Then the homogenate was transferred to a 2-microliter-protein gel sample buffer. Samples loaded on to the gel was run at 100 v and stained with comassie blue; observations were made next lab. (Clendening 2014)…
The purpose of this experiment is to see whether or not temperature plays a role in the percent change during diffusion or osmosis.…
The overall goal of experiment four was to determine the identity of unknown cations presented to the student. But in order to know the identity of these unknowns, in part 1, Ag+, Pb+, and Hg22+ were presented to the student in aqueous solutions and then precipitated through experimentation. In part 2, the same procedure was enacted to determine which substances precipitated through qualitative analysis. Solubility rules were also a major theme as solubility is important in determining whether a reaction will produce a precipitate.…
1. Some potatoes increased in mass while others decreased due to osmosis. Potatoes that had a higher concentration increased in mass because water passed through the membrane into the potato in an effort to dilute it. Potatoes that had a lower concentration than the solution decreased in mass because water flowed through the membrane into the solution trying to dilute the solution. The water moves to the higher concentration to dilute it/maintain homeostasis, increasing or decreasing mass depending on whether it goes in or out.…
The number of electrons forming a charge cloud around the nucleus is (pick one of the following) greater than; equal to; smaller than the number of protons in the nucleus of the atom.…
Enzymes are biological molecules that catalyze chemical reactions. In enzymatic reactions, the molecules at the beginning of the process, called substrates, are converted into different molecules, called products. Almost all chemical reactions in a biological cell need enzymes in order to occur at rates sufficient for life. Like all catalysts, enzymes work by lowering the activation energy for a reaction, thus dramatically increasing the rate of the reaction. As a result, products are formed faster and reactions reach their equilibrium state more rapidly. Most enzyme reaction rates are millions of times faster than those of comparable un-catalyzed reactions. As with all catalysts, enzymes are not consumed by the reactions they catalyze, nor do they alter the equilibrium of these reactions. However, enzymes do differ from most other catalysts in that they are highly specific for their substrates. Enzymes are known to catalyze about 4,000 biochemical reactions.…
For the test tube with the ratio of acetylene to air is 1:1, a flame traveled down a test tube, a slight pop and black residue was left…
3 Watch each part of the experimental demonstration and make preditions about wht will happen in each scenario . Record your preditions and observations in the Data an Observations section of your labatory report format.…
Lichens are a sybiotic association of fungus (mycobiont) and algae (Pediastrum boryanum . The fungus engulfs the algae, then supplies carbs, nitrogen, and vitamins while the algae photosynthesizes. This unusual relationship enables the lichen to grow in environments where neither fungus nor algae would normally be able to solely survive. They are found on every continent, ranging all the way from the freezing Arctic to the sweltering grasslands of Africa. Even though they can live in such extreme conditions, they do require clean air. This is why they are excellent environmental indicator. An indicator species is an organism whose presence or absence reflects the overall health of an environment. Lichens flourish where there is good air quality; however, they cannot survive where the air is polluted and toxins loom. This is because they have no stomata, or pores, to shut to keep the toxins out; therefore, absorbing all of the pollutants in the air. Because they are such unique organisms, they accumulate different metal anions in the air than other organisms, like plants do, for example. Lichens attract toxic metal ions. An ion is a charged atom. If the ion has a positive charge, it is a cation. An ion with a negative charge is an anion. During the ion exchange process, Hydrogen, an anion, is released and metal cations move in. This process occurs when lichens are exposed to polluted air. When the lichens are dropped into any of the metal solutions, their cell wall releases the methylene blue ions, which are replaced by metal cations. Upon their detachment, the ions integrate into the solution, which in turn, colors it blue. The darker the solution, the more metal cations taken in by the lichen. Valence is a measure of how much an atom wants to combine with something else. If the valence of a metal ion affects lichen uptake, then the higher the cation, the…
2. Explain how to focus a microscope using the high power lens. Include any safety issues you need to be aware of.…
Yes. My hypothesis was the chocolate bar will gradually decrease in density as it gets smaller. According to the chart below, not only did the density decrease, but the mass and volume decreased as well. To reiterate, my hypothesis agreed with the results of my experiment.…
groups. It was conducted to further the research in choice overload and paralysis of the mind.…
It functions to help control the amount of water that is permeable to the walls of the collecting duct, meaning the hormone helps make urine more concentrated. The main factor stimulating the release of ADH is the osmolarity in the blood; which is the concentration of solutes outside of cells. Typically, ADH is released under conditions of elevated osmolarity above a certain level and not under lower osmolarity conditions. This increase in osmolarity is detected by pressure receptors (osmoreceptors) in the hypothalamus. Once released, ADH makes its way to the kidneys where it is able to bind to receptor proteins on cells in the collecting ducts of nephrons. ADH allows water to leave the collecting duct and be put back into circulation. On the cellular level, ADH promotes more aquaporins to be put into the membrane of the collecting duct. This occurs when ADH binds to the receptors mentioned earlier. This binding sends a signal for the cell to produce more aquaporin-2 (AQP-2), a protein known as an ADH-regulated water channel. With more channels available in the apical membrane, water is able to diffuse out of the cell to reduce osmolarity. The overall effect of this hormone is to put water back into circulation to lower the osmolarity of the blood to return the body to homeostasis. The increased osmolarity may be caused dehydration (also increases heart rate) or other things. In that case, ADH helps to return heart rate, water and ion levels to homeostatic…
A hydrated crystal or hydrated occurs when water becomes tightly attracted to a metal salt base on it’s polarity. The water molecules maintain integrity as molecules, however they are considered to be part of the formula of the hydrate. When the hydrate metal salt crystal is heated, the attractions to the water are broken by the heat energy and the water escape from the crystal. After heating the salt crystal is called as anhydrous, which means without water. Many of the salt contain transition metals such as cobalt that is colorful (purple). Often the color of transition metal like cobalt’s color will change as a function of how many waters it is attracted to. Compounds containing water (H2O) of hydrated are written with water separate by a dot (.). This (.) means for hydrated metal salt there are how many number of moles of water bound to each mole of that metal salt.…
The purpose of this lab is to investigate interference, otherwise known as the diffraction of light. A beam of light acts a wave, and we are able to use equations so calculate the wavelength of the light used. The diffraction of a straight edge demonstrates that light waves bend around straight edges, allowing light to enter an area of shadow. When waves are superposed, they reinforce each other when crests are in phase and cancel out when they are not in phase. An image projected by light waves shows this by producing concentric circles of light. When slits are used, the laser superposes itself and creates constructive interference. The resulting spots are measured to give data that allows one to find the wavelength using the equation λm = dsin θ. These diffraction and interference with such slits was successful because of low percent errors in the wavelengths calculated. Additionally, a diffraction gradient creates better-defined spots, and more accurate measurements, yielding the lowest uncertainties.…