Laboratory Exam 1
Microbiology 1214
A partial study guide only. Not intended to replace the review sheets or the “Know the following” sections on your labs.
These slides are not presented in any particular order.
Name this
Procedure
Identify the two types of bacteria present by shape and gram stain.
In a gram stain what is the primary stain?
The mordant? The counterstain?
How does this differ from a simple stain?
Identify the shape and gram stain of the bacteria present in this gram stain.
Identify the shape and gram stain of the bacteria pictured. Think on this one!
Name the gram stain and shape of the bacteria pictured.
Name the genus of the organism pictured.
Note the size when determining the organism.
Identify this type of agar. We use this agar to check for _______.
Name This Technique.
What is its purpose?
What type of hemolysis is pictured below?
Identify the Following
I go straight from the 4x objective to the 100 x objective? Why?
Identify the
Following
Identify the staining procedure pictured. Identify what we are doing here.
What type of plate would we be making using this technique?
Name the Structure
Pictured.
What Settings Do We
Use to Sterilize Bacteria
Using this Instrument?
What temperature do we use to sterilize?
At what temperature does agar melt?
At what temperature does agar solidify?
At what temperature do we prefer to work with liquified agar in the lab?
Name this Technique
Name this Organism. How does it reproduce? What is another name for a bud?
In the capsule stain, what is the primary stain?
What is used to stain the background? What is the method used to make the smear called?
What is an advantage to not heat fixing?
What is a disadvantage to not heat fixing?
• In the capsule stain, what is the acidic dye used in the procedure?
• What is the basic dye used in the procedure. Identify the three types of flagella pictured. What is the difference between true
bacterial