Light Intensity Lab Report

Light Intensity Experiment: For the light intensity experiment, our first set of data illustrated strange correlations between the three tubes placed in front of a light source, so the data provided in e-campus was used. The tube that was 24 cm away from the light had the greatest decrease in absorbance over the course of 20 minutes, followed by the 30 cm and then the 49 cm light apparatus. The tube that was placed in the dark (wrapped in foil) remained a dark blueish-green color whereas the tubes that were exposed to the light became a lighter shade of green when exposed to the light.
Action Spectrum/Floating Disk: The results of our action spectrum experiment illustrated that red light (700 nm) and blue light (450 nm) produced the most floating
When it is oxidized DPIP appears blue, but when it accept an electron it is reduced and changes to a clear color , a definite sign that a reaction is occurring. The spectrophotometer measures the absorbance of solutions by passing light through a sample. A solution with a more concentrated color will have a higher absorbance number such as in the dark, 30 cm , and 49 cm tubes. These tubes had a more concentrated color due to the fact they were not absorbing as much light, therefore the photosynthetic reaction was not reacting as quickly as the 24 cm tube that was much closer to the light. In contrast, a solution with a clearer color will have a much lower absorbance number such as in the 24 cm tube. Based on the evidence from the data, this supports our hypothesis that more light exposure will result in a decrease in the absorbance. Our first set of results showed a strange correlation between data. Instead of the 24cm tube having the lowest absorbance , our data showed ( in increasing absorbance order) the 30 cm tube followed by the 49 cm, 24 cm, and dark tube. This result was very unexpected. A possible cause for this is the fact another table got their tubes confused with ours, essentially corrupting our table’s