Liver Homogenate Lab Report

Discussion: The liver homogenate was expected to have the highest amount of protein concentration, DNA concentration, and total glucose when compared to the other homogenates. The liver was the biggest organ in B. taurus and it carried out numerous functions such as producing bile, synthesizing proteins for blood clots, and recycling old red blood cells. The liver ended up containing the most DNA concentration, but it was surpassed in protein concentration by the kidney. The protein concentration data could have been skewed by the handling of the liver homogenate throughout the experiment. Although each homogenate was placed on an ice box, there is a possibility that the temperature increased as the homogenate was added to other reagents, causing proteins to denature in the process. The DNA concentration was the highest in the liver homogenate because there is a constant need for replication of liver cells in order to keep the liver’s function operating (Table 1). Similarly, the total glucose was the
Although it was not expected, the kidney had the highest concentration of protein of the homogenates. The high protein concentration is warranted because it has to filter nitrogenous wastes and maintain homeostasis continuously despite the smaller size. Without the high protein concentration, B. taurus could not filter out wastes properly, which can cause harm to the organisms. The kidney unexpectedly contained the lowest amount of total glucose, which showed that the small size of this organ does not require a large amount of glucose in order to keep functioning (Figure 2). The DNA concentration was expected to be the second highest, but there appeared to be more DNA concentration than expected because of its size. The kidney most likely needed to replicate DNA quickly because cells were damaged by the waste that entered it (Table