Malaria Diagnostic Test Lab Report
Description for Using Malaria Rapid Diagnostic Test (mRDTs)
The procedure of the test as contained in the manufacturer’s instructions as approved by the National guidelines for diagnosis and treatment of malaria, (FMoH, 2011) will be followed. its reliability was futher proven by Olaniyan, et al., (2013). The RDT provided for the study has LOT number and expiry date which must be recorded. Preferably RDT of known quality assurance will be used. The device usually contains a cassette, an applicator loop stick, lancet, a buffer solution and desiccant. The cassette has two wells on the surface. The labeling of the patient I.D no. will be done on the RDT cassette using glass marker and placed on a smooth flat dry surface. 5 µL of blood (one loop-full) …show more content…
On the frosted end of the clean grease-free slides, the identification number and date for each participant would be written using a glass marker. A 12µl of blood would be spread over a diameter of 15mm for thick blood film, while 2µl of blood would be used for a thin blood film on the same slide using an automated micropipette. Another “spreader” slide will be held with polished edges at a 450 angle at the edge of the blood drop and then pulled forward to make an even spread with a tail end. The thin film will fixed in absolute methanol for 2 seconds to prevent lysis of the red blood cells and air dried on the rack (WHO, 2000). The dried slides will be kept in the incubator already set at 350C for 48 …show more content…
The stain will be left for 45 minutes and then gently rinsed off with buffered solution of pH 7.2 until the flooding becomes clear to sight (WHO, 2000). The slides will be placed vertically on the slide rack and allowed to drain and dry and later arranged into the slide
The procedure of the test as contained in the manufacturer’s instructions as approved by the National guidelines for diagnosis and treatment of malaria, (FMoH, 2011) will be followed. its reliability was futher proven by Olaniyan, et al., (2013). The RDT provided for the study has LOT number and expiry date which must be recorded. Preferably RDT of known quality assurance will be used. The device usually contains a cassette, an applicator loop stick, lancet, a buffer solution and desiccant. The cassette has two wells on the surface. The labeling of the patient I.D no. will be done on the RDT cassette using glass marker and placed on a smooth flat dry surface. 5 µL of blood (one loop-full) …show more content…
On the frosted end of the clean grease-free slides, the identification number and date for each participant would be written using a glass marker. A 12µl of blood would be spread over a diameter of 15mm for thick blood film, while 2µl of blood would be used for a thin blood film on the same slide using an automated micropipette. Another “spreader” slide will be held with polished edges at a 450 angle at the edge of the blood drop and then pulled forward to make an even spread with a tail end. The thin film will fixed in absolute methanol for 2 seconds to prevent lysis of the red blood cells and air dried on the rack (WHO, 2000). The dried slides will be kept in the incubator already set at 350C for 48 …show more content…
The stain will be left for 45 minutes and then gently rinsed off with buffered solution of pH 7.2 until the flooding becomes clear to sight (WHO, 2000). The slides will be placed vertically on the slide rack and allowed to drain and dry and later arranged into the slide