Caffeine is a stimulant which increases cardiac arrhythmia (improper heart rate) by increasing stress hormone (e.g. adrenaline) secretions. It also increases blood pressure which contributes to the blockage of receptors such as adenosine A1 and A2 receptors. The caffeine blocking receptors also blocks vasodilatory function, in which blood vessels become less dilated. Hence wise, caffeine is also known as a mild stimulant of the central nervous system and also stimulates the cardiac muscle and smooth muscles. Caffeine increases levels of neurotransmitters such as norepinephrine has been shown to increase the metabolic rate.
For this experiment I believe that the cardiac arrhythmia will increase as the concentration of the caffeine increases. I also believe that the three the reading of the systolic pressure with distilled water will increase as distilled water contains less oxygen in comparison to normal water. This means the heart will have to beast faster in order to obtain oxygen for cells.
Firstly, on a cavity slide, we placed a few strands of cotton wool to make sure the daphnia's do not move out of focus once they are on the slide. Using a pipette, we transferred 1 daphnia onto the cotton wool. Whilst transferring the daphnia, we transferred some of the original water from the natural habitat across. Measuring the heart rate using the pond water allows us to compare the 3 other fluids to the control fluid and compare the heartbeats.
We then placed the slide on the microscope stage and made sure we used the lowest setting of the light. This is to make sure that we did not cause a sudden cardiac arrhythmia and break the ethical values that we considered at the beginning of the experiment. We switched on the light and started to focus on the daphnia; then we changed the light intensity so the daphnia appears dark. Then, we located the heart and its digestive tract. We measured the heartbeat