Methylene Blue Lab Report
OXYGEN BREWERS PLATE:
For this test a nutrient agar plate was streaked with the unknown organism and placed into the anaerobic jar/GasPak anaerobic system and incubated at 37 degrees celcius. This system consists of a polycarbonate jar, a lid with a gasket to prevent airflow, a strip infused with methylene blue, and a pouch containing sodium borohydride, sodium bicarbonate, citric acid, and a palladium catalyst. When water is added to the pouch, the sodium borohydride, sodium bicarbonate, and citric acid react to form hydrogen and carbon dioxide. The palladium catalyzes a reaction between the hydrogen and the oxygen within the jar; this reaction creates water, which forms as condensation on the inside of the jar. Methylene blue is blue in
For this test a nutrient agar plate was streaked with the unknown organism and placed into the anaerobic jar/GasPak anaerobic system and incubated at 37 degrees celcius. This system consists of a polycarbonate jar, a lid with a gasket to prevent airflow, a strip infused with methylene blue, and a pouch containing sodium borohydride, sodium bicarbonate, citric acid, and a palladium catalyst. When water is added to the pouch, the sodium borohydride, sodium bicarbonate, and citric acid react to form hydrogen and carbon dioxide. The palladium catalyzes a reaction between the hydrogen and the oxygen within the jar; this reaction creates water, which forms as condensation on the inside of the jar. Methylene blue is blue in