Microfluidics Device Testing Lab Report

2. Microfluidics-device testing In this step, we use 10 µm polystyrene beads as a cell for testing the trapping ability of the device. The polystyrene beads were mixed with deionized water (DI water) and surfactant (Tween 20) in the ratio of 100: 1: 10 v/v/v. The fast green FCF (C37H34N2O10S3Na2) was added to the mixing solution for increased the particle visualization and then the solution was containing into a 10 ml syringe that connected to the device at an inlet. Before start the experiment, the microfluidics-device was cleaned and removed retained air bubbles by injection of the mixing solution between DI water and Tween 20 (ratio of 1:10 v/v) at flow rate of 0.5 ml/h. Afterward, the polystyrene bead solution was suctioned into the device by using the flow rate of 0.1 ml/h for 10 minutes continuously then the retain particles and air bubbles were removed by the mixing solution (same as usage before experiment) at flow rate of 0.5 ml/h. The occupied microwells were counted at the front, middle, and back part of the device that have the most captured particles in each area (117 microwells per area) for evaluate the trapping efficacy.

3. The usage of microfluidics-device for cell trapping
Canine leukocytes from blood samples were used as a target cells. Blood samples collected from dog patients in a small animal hospital, faculty of veterinary science, Chulalongkorn University during August to September, 2015. The criteria for blood sample collection are total 60 dogs, which hematocrit value range of 40-50% (similar to normal range to avoid the bias of size and physiological of white blood cells), any breeds, sex, age, and, diseases then the dogs were separated into 3 groups (20 dogs for each group), 1 ml of blood was collected from each dog.