In this lab we were asked to observe changes and colors when mixing two or three chemicals together. The actual experiment itself used twelve different pipits and three chemicals I selected from my house. To begin, I first gathered everything, including the 96 well plate, 24 well plate, goggles, and gloves along with the observations of chemical changes bag. I set it up all on the the kitchen table that had paper towels on it. Next, I made my data table so that I could record my reactions. Third, I sat all the pipets in the 24 well plate. At this point, I began the lab by following the instructions for each chemical. I had to drop two drops of the first chemical into one well, followed by two drops of the second chemical into the same well. In some instances the second chemical was only one drop and there was also one where we added a third chemical. Once this was done I placed the well plate over white paper and then over black paper and made an observation of what happened when it was over each piece of paper. For each reaction, I wrote down the chemicals used, the well plate number I used, changes I noticed immediately, and then what I noticed with the well plate over the white and black pieces of paper on my data table. After completing the list of chemicals used together, I took three cleaning products from my home and mixed the Bromthymol Blue with them. I used the same format two drops of cleaning products and two drops of Bromthymol Blue. I added them to the data table that I had already created. After I was done with the experiment I followed the safety features and cleanup methods listed in our lab manual. At this point I realized why we had to have distilled water.
Data Table 1 – Observations of Chemical Changes, using chemicals supplied
Well # Chemicals Used Reaction Paper Change A1 NaHCO3 and HCl - C02 white bubbles formed more noticable when placed on black paper
B1 HCl and BTB turned bright yellow/orange darker