Optimum Temperature Of Sucrase

For this experiment, there will be an optimal point for each factor. I believe that the optimal temperature for the enzyme sucrase will be 37°C because that is the point where the temperature increases the rate of reaction to its greatest point without denaturing it. For the pH test, I think that the optimal pH for sucrase will be the pH of 7 because it is neutral so it won’t affect the charge in a negative or positive way. For the denaturation test, I think that the optimal treatment for the enzyme is when both the enzyme and substrate are untreated. For the substrate concentration test, I think that the optimal concentration is 100% because the more the substrate, the faster the enzyme can bind to it. Based on the data above, all four hypotheses were proven correct. Based on the graph and data table, the optimal temperature for sucrase was 37°C because it had a reactivity score of 4, meaning it was very reactive. For the pH test, based on the graph and data table, the optimal pH was in fact ranged from 3-7. This means that any pH from 3-7, gives the highest rate of reaction to sucrase. The denatured test was proven correct because as the hypothesis said, if the enzyme and substrate are both untreated, the rate of reaction will be the highest and based on the graph and data table, this was the case.

Lastly, the hypothesis for the substrate concentration was proven correct because based on the graph and data table, a substrate concentration of 100% gives a reactivity score of 4, meaning it was most reactive.

When looking at the results of the lab, we are confident that the hypotheses were proven correct. All of the intended changes based on prior knowledge as well as the results occurred. All of the necessary changes in color occurred for the right changes in temperature, pH, denaturation, and substrate concentration. If this lab occurs again, there can be more accuracy if there are more data sets as well as more trials for each of the factors to ensure accuracy and precision of the results and to see if the relationship between each individual factor and enzyme-substrate complex still occurs.

The real world significance for enzymes is enormous. As said above, all organisms need enzymes in order to live and thrive. In the human body, there are many enzymes that have different functions like breaking down large macromolecules to DNA transcription and translation through digestion in the stomach and small intestine. It is important to know what affects enzymes in our body because temperature and pH are factors that pay a large role in our system and it is import that we are aware of what are the optimal changes for specific enzymes and what can denature them. Enzymes also play a huge roll in agriculture. Without enzymes, we would not have any break, alcohol, or milk. Enzymes speed up chemical reactions in order for these products to occur.