Osmosis And Diffusion Lab Report
Introduction: Diffusion and osmosis are passive processes of transport. Passive transport involves no disbursement of energy by the cell. Diffusion movement is from high concentration to low concentration, which the driving force for this type of movement is kinetic energy particles themselves. Which crystal (Methylene blue, solid or Potassium Permanganate KMnO4-purple) will move further than the other due to the driving force (kinetic energy)? My prediction is that Potassium Permanganate KMnO4- purple (molecular mass- 158.03 g/mol) will move further than Methylene blue, solid (molecular mass- 319.85 g/mol) since it weighs less. In osmosis is diffusion of H2O across a selectively permeable membrane (dialysis tubing). There is only movement of water, not solute, across the plasma membrane. Water moves from a solution to a lower solute to prevent a passage of water across an osmosis membrane, which is known as osmotic pressure. Osmotic pressure increases with increasing solute concentrate. What will be the changes of weight when a 10 mL tap water bag is placed into Beaker #1, which is filled to 200 mL of tap water? My prediction is that there will be no change in the weight. Since solutions that contain the same concentrate of solute particles per volume of a solution that than within the cells are said to be isotonic solutions, which means the concentration of a solute particles is the same on
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both sides . What will be the changes of weight when a 10 mL of 20% sucrose solution bag is placed into a 200 mL beaker of tap water? My prediction is that there will show a weight increase. Solutions that contain a solute concentration less than that within a cell are said to be hypotonic, which means the water moves into cell, fewer solute particles per volume of solution outside than inside . What will be the change of weight when a 10 mL of 40% sucrose solution bag is placed into a 200 mL beaker of tap water? My prediction is that there will show a weight increase. What will be the change of weight when a 10 mL of 60% sucrose solution bag is placed in a 200 mL beaker of tap water? My prediction is that there will also be a weight increase. What will be the change of weight when a 10 mL of tap water bag is placed into a beaker of 200 mL of 60% sucrose solution? My prediction is the weight will decrease. Solutions that contain higher concentrate of solute particles per volume of solution that within the cell are said to be hypertonic, which is a concentration of solution is greater outside the cell then inside. There is high water inside the bag so water will come out .
Materials:
Part A:
Methylene blue, solid
Potassium Permanganate KMnO4- purple
Carolina Plastic ruler- 6 inches (metric)
Forceps
Petri dish with agar gel (premade)
Timer on Galaxy S2 phone
Method:
Part A
1.
The correct petri dish with premade agar gel was collected and the lid was removed.
2. The lid of Potassium Permanganate KMnO4 was placed aside.
3. Placing one crystal of Potassium Permanganate KMnO4- purple from the bottle and into the petri dish leaving space for it to
expand.
4. The timer has been started immediately.
5. The bottle Methylene blue, solid was collected and the lid was removed, placing it aside.
6. Placing one crystal of Methylene blue, solid from the bottle into the petri dish leaving space for it to expand and not touching Potassium Permanganate KMnO4.
7. After waiting 50 minute, now measuring the Potassium Permanganate KMnO4 and then recorded size.
8. After waiting 50 minute, now measuring the Methylene blue, solid and then recorded size.
Results:
(On graph paper)
Conclusion:
After the experiment was over and discussing the data, we were able to configure the data and research to determine that the dialysis were cells. Therefore, when dropping the crystal into the petri dish, the crystal molecules diffuse into the agar gel down the concentration gradient, oxygen then diffuses into the cell and carbon dioxide diffuses out of a cell. The rate of diffusion depends on the molecular weight of the chemical , so concluding that for Part A experiment the data supports the hypothesis that I have made since Potassium Permanganate KMnO4’s molecular weight is less than Methylene blue, solid. The net movement of water by osmosis is dependent upon the concentration gradient between the cytosol and the solution in which the cell is immersed. Water continues to move until equilibrium is reached (the concentration of water in the cell surrounding fluid are equal). For beaker #1 (Part B) it was an isotonic solution so there was no change in the weight, my hypothesis for this beaker was supported by my data. Steepness of a concentration gradient is a measure of the difference in concentration of a substance between two areas. A steeper concentration gradient causes a faster rate of diffusion . In beaker #2 (Part B) there was 80% extra cellular fluid. In beaker #3 (Part B) there was 60% extra cellular fluid. And in beaker #4 (Part B) there was 40% extra cellular fluid, which this beaker had the steeper gradient causing the most weight gain. My data for Part B had supported the hypothesis that I had made for each of these beakers. Lastly, beaker #5 (Part B) was hypertonic, high water was inside the bag so water will come out and the weight decreased, by the show of my data it had supported my hypothesis.
Citations:
Human Anatomy and Physiology I Supplement Laboratory Materials, pg.44 and pg.45, WCCC Biology Department,2013 http://sciweb.hfcc.net/biology/jacobs/bio131/diffusion/diff&os.html, Jacobs, 1998
Human Anatomy & Physiology, McKinley, 2013
both sides . What will be the changes of weight when a 10 mL of 20% sucrose solution bag is placed into a 200 mL beaker of tap water? My prediction is that there will show a weight increase. Solutions that contain a solute concentration less than that within a cell are said to be hypotonic, which means the water moves into cell, fewer solute particles per volume of solution outside than inside . What will be the change of weight when a 10 mL of 40% sucrose solution bag is placed into a 200 mL beaker of tap water? My prediction is that there will show a weight increase. What will be the change of weight when a 10 mL of 60% sucrose solution bag is placed in a 200 mL beaker of tap water? My prediction is that there will also be a weight increase. What will be the change of weight when a 10 mL of tap water bag is placed into a beaker of 200 mL of 60% sucrose solution? My prediction is the weight will decrease. Solutions that contain higher concentrate of solute particles per volume of solution that within the cell are said to be hypertonic, which is a concentration of solution is greater outside the cell then inside. There is high water inside the bag so water will come out .
Materials:
Part A:
Methylene blue, solid
Potassium Permanganate KMnO4- purple
Carolina Plastic ruler- 6 inches (metric)
Forceps
Petri dish with agar gel (premade)
Timer on Galaxy S2 phone
Method:
Part A
1.
The correct petri dish with premade agar gel was collected and the lid was removed.
2. The lid of Potassium Permanganate KMnO4 was placed aside.
3. Placing one crystal of Potassium Permanganate KMnO4- purple from the bottle and into the petri dish leaving space for it to
expand.
4. The timer has been started immediately.
5. The bottle Methylene blue, solid was collected and the lid was removed, placing it aside.
6. Placing one crystal of Methylene blue, solid from the bottle into the petri dish leaving space for it to expand and not touching Potassium Permanganate KMnO4.
7. After waiting 50 minute, now measuring the Potassium Permanganate KMnO4 and then recorded size.
8. After waiting 50 minute, now measuring the Methylene blue, solid and then recorded size.
Results:
(On graph paper)
Conclusion:
After the experiment was over and discussing the data, we were able to configure the data and research to determine that the dialysis were cells. Therefore, when dropping the crystal into the petri dish, the crystal molecules diffuse into the agar gel down the concentration gradient, oxygen then diffuses into the cell and carbon dioxide diffuses out of a cell. The rate of diffusion depends on the molecular weight of the chemical , so concluding that for Part A experiment the data supports the hypothesis that I have made since Potassium Permanganate KMnO4’s molecular weight is less than Methylene blue, solid. The net movement of water by osmosis is dependent upon the concentration gradient between the cytosol and the solution in which the cell is immersed. Water continues to move until equilibrium is reached (the concentration of water in the cell surrounding fluid are equal). For beaker #1 (Part B) it was an isotonic solution so there was no change in the weight, my hypothesis for this beaker was supported by my data. Steepness of a concentration gradient is a measure of the difference in concentration of a substance between two areas. A steeper concentration gradient causes a faster rate of diffusion . In beaker #2 (Part B) there was 80% extra cellular fluid. In beaker #3 (Part B) there was 60% extra cellular fluid. And in beaker #4 (Part B) there was 40% extra cellular fluid, which this beaker had the steeper gradient causing the most weight gain. My data for Part B had supported the hypothesis that I had made for each of these beakers. Lastly, beaker #5 (Part B) was hypertonic, high water was inside the bag so water will come out and the weight decreased, by the show of my data it had supported my hypothesis.
Citations:
Human Anatomy and Physiology I Supplement Laboratory Materials, pg.44 and pg.45, WCCC Biology Department,2013 http://sciweb.hfcc.net/biology/jacobs/bio131/diffusion/diff&os.html, Jacobs, 1998
Human Anatomy & Physiology, McKinley, 2013