Guiding Question: Are some laundry detergents better than others?
Purpose: We intend to test if the cost of a detergent affects its quality but putting 3 drops of Jell-O into 25mL of Jell-O and examining how much of the Jell-O dissolves.
Hypothesis: If the cost of the detergent increases, then the amount of Jell-O dissolved will increase by 0.3 cm.
Materials/Equipments: * 4-1mL Pipettes * 5cm long standard drinking straw with a diameter of .6cm. * Jell-O brand gelatin in lime * Insulated pad * 5-10mL of distilled water * A clear, flat, metric ruler * 1 pair of standard cutting scissors * 1 blank printer paper * 1 Crucible Tongs * 1 medium point black or blue sharpie * 2 1L Beaker * Hot plate * …show more content…
Stirring Rod * 4 Petri dishes -8.9cm in diameter * 1 Standard refrigerator * 1 Celsius thermometer * 250mL graduated cylinder * 25mL graduated cylinder * 10 Paper towels * Flat surface of at least 40cm by 40cm * Sink * #2 Pencil with eraser * Tooth pick of 6.5cm in length * 91% Isopropyl alcohol * Card board box * 4 Test tubes * 1 Test tube rack * 1 Test tube brush * 3 sheets of lined paper for data
Procedures: 1. Gather your Jell-O, distilled water, 1 L beaker, hot plate, stirring rod, Petri dishes, thermometer, 250mL graduated cylinder, and 25 mL graduated cylinder. 2. Measure 471 mL of distilled water into the 1 L beaker, set beaker on hot plate, and turn hot plate to #10 heat setting. 3. Heat water until boiling. Water should be above 100 degrees C; this takes about 10 minutes. 4. Remove beaker from heat and place on an insulated pad, add 2 jello packets, and mix with stirring rod. 5. Measure 471 mL of cool distilled water using a 250 mL graduated cylinder. Add the cool water to the boiling jello solution. 6. Then, measure 25 mL of the jello solution into the bottom of each Petri dish. Cover immediately to prevent evaporation. 7. Carefully stack Petri dishes and refrigerate overnight. 8. In the morning, take out your test tube rack, 4 test tubes, Gain detergent, Ecos detergent, Tide detergent, and your 4 pipettes. 9. Place the test tubes into the test tube rack at a 90 degree angle, in separate wells, with the open sides of the tubes facing upwards. 10. Take your Gain detergent and pour it into the first test tube so that it fills up the tube 3cm, then place it into the first well from the left in the test tube rack. 11. Take your Ecos detergent and pour it into the open side of the second test tube so that it fills up the tube 3cm, then place the tube into the second well from the left, of the test tube rack, with the open side still facing upward. 12. Take your Tide detergent and pour it into, the open side of the third test tube so that it fills up the tube 3cm, then place the tube into the third well from the left, of the test tube rack, with the open side still facing upward. 13. Now place three of the pipettes into each of the three test tubes with the smaller, thinner part of the tube in the detergents. 14. Now take your clean 1L beaker and fill it half way with distilled water. Place the last pipette into the water with the small, thin part of the pipette in the water. 15. Place these things to the side, out of the way; they will be used later on. 16. Remove your petri dishes from the fridge; begin careful to hold the petri dishes containing jello on the sides of the dish to prevent melting the jello. Then place 1 petri dish on a blank sheet of printer paper and set the others on the side, not stacked and apart from each other. 17. Trace the petri dish onto the paper and slide the dish off of the paper onto the side with the other dishes. 18. Using your clear ruler and pencil, draw 2 lines on your tracing that divide the tracing into 4 even quadrants. Keeping in mind that the diameter is 8.9cm. 19. Put a circle or dot in each quadrant 2.5cm away from the center where your 2 lines cross, making sure to evenly place the dots in the center of the quadrants. 20. Place the petri dish back on top of your tracing and remove the lid from the dish. 21. Get your 5cm long straw with a diameter of .6cm and insert the straw into the jello at a 90 degree angle above one of the dots in your tracing. Make sure that you allow the dot to be in the center of the well you are making with your straw, by looking through the straw as you puncture the jello. 22. After inserting the straw into the jello, twist the straw in a clock-wise motion completing the circle at least twice, while holding the base of the dish to keep it from turning along with the straw. 23. Vertically lift the straw out of the jello. 24. If a jello circle appears inside of your straw after removing your straw from the petri dish, then take your toothpick and use it to pierce the jello in the straw and remove it. Then move to step 20. If this does not happen to you then move onto step 17. 25. Now grab your toothpick and insert it into the jello at a 90 degree angle on the edge of the circle you have created with your straw in step 14. Carefully trace the circle in the jello made by your straw, in a clockwise position. Trace the circle at least 3 times around. 26. Remove your toothpick from the jello and pierce into the center of the small circle of the jello you have just created. 27. Remove the toothpick along with the small jello circle with should now be attached. Dispose of the jello circle in the sink. If there is still some jello left in the well you have made, remove the excess with the toothpick, and dispose of that in the sink as well. 28. Repeat steps 12-19, 3 more times for this petri dish. 29. Repeat steps 12-20 for your next 3 dishes. 30. Put the lids back on your dishes and get out your sharpie. Uncap the sharpie and draw a horizontal line on the side of the lid. Them life the lid up and draw another horizontal line on the edge of the dish to correspond with the line you drew on the lid. 31.
Place the lids back on the dishes and match up the line on the base of the dish as with the line on the lid. 32. With your sharpie still uncapped, label above and to the right of each well, 1, 2, 3, or 4. Do not repeat numbers on a single petri dish. Do this to each of your dishes. 33. Now, take one petri dish, placing it on your paper and let the numbers face right side up. Along the bottom of the lid, write ‘Gain-A’. This label will be used to remember what the dish is containing. And place that dish to the side, holding the petri dish properly. 34. Take another dish that is unlabeled and place it on your paper, holding the petri dish by its sides to prevent from melting the jello. And along the bottom of the lid write ‘Ecos-H’. This again will be used to remember what the dish is containing. Now place this dish to the side, holding the petri dish the proper way. 35. Take another dish that is unlabeled and place it on your paper, holding the petri dish by its sides to prevent from melting the jello. And along the bottom of the lid write ‘Tide-G’. This will be used to remember what the dish is containing. Place this dish to the side, holding the petri dish the proper
way. 36. Take the last petri dish that is unlabeled and place it on your paper, holding the petri dish by its sides to prevent from melting the jello. Along the bottom of the lid write ‘Control’. This will be used to remember what the dish is containing. Control refers to the control variable, which is water. Place this dish to the side with the other dishes, holding the dish the proper way. 37. Now gather your 1L beaker of distilled water and the test tube rack with the detergent filled test tubes in the rack, along with the pipettes that were inside of the tubes and the beaker. Place this behind your petri dishes and line up your four dishes in front of the rack. 38. Order your dishes according to the order of the detergents in the test tubes in the rack. The order of the tubes in the rack is Gain, Ecos, and then Tide, from left to right. Then place the beaker of distilled water to the right of the test tube rack. And the control petri dish in front of that beaker. 39. Remove the lid from the Gain petri dish, and set the lid to the side. 40. With the small, thin, open part of the pipette still in the detergent, squeeze the thick part so that the detergent fills the pipette at least 2cm high. Then release your squeezing hold on the thick part of the pipette and pull it out of the test tube. 41. Suspend the pipette above the test tube so that if there is any dripping, it goes back into the tube. When the dripping, if any, stops, move the pipette to the petri dish and hover the small part of the pipette over the first well in the jello. 42. Making sure that the small, open part of the pipette is directly over the well, carefully squeeze the thick part of the pipette so that a single drop of detergent falls into the well. Do this until only a total 3 drops are in the first well. 43. Move over to the second well and repeat step 42. Do this to the third and fourth well too, then return the pipette to the test tube. If you run out of detergent in the pipette while inserting the detergent drops, then repeat step 40 and then return to where you were. 44. Put the lid back on the Gain petri dish and put it to the left of the test tube rack, out of the way. 45. Remove the lid from the Ecos petri dish and set the lid to the side. 46. Repeat steps 40-43 then put the lid back on the dish and while holding it properly by its sides, place the dish on top of the Gain petri dish to the left of the test tube rack. 47. Remove the lid from the Tide petri dish and set the lid to the side. 48. Repeat steps 40-43 then put the lid back on the dish and while holding it properly by its sides, place the dish on top of the Ecos petri dish to the left of the test tube rack. 49. Remove the lid from the Control petri dish and set the lid to the side. 50. With the small, thin, open part of the pipette still in the distilled water, squeeze the thick part so that the distilled water fills the pipette at least 2cm high. Then release your squeezing hold on the thick part of the pipette and pull it out of the beaker. 51. Suspend the pipette above the beaker so that if there is any dripping, it goes back into the tube. When the dripping, if any, stops, move the pipette to the petri dish and hover the small part of the pipette over the first well in the jello. 52. Making sure that the small, open part of the pipette is directly over the well, carefully squeeze the thick part of the pipette so that a single drop of water falls into the well. Do this until only a total 3 drops are in the first well. 53. Move over to the second well and repeat step 43. Do this to the third and fourth well too, then return the pipette to the test tube. If you run out of detergent in the pipette while inserting the distilled water drops, then repeat step 40 and then return to where you were. 54. Place the lid back on the control petri dish and take out the clear, flat ruler, and place it on top of the white printer paper so you can see the numbers of the ruler. 55. Line up the edge of the well labeled 1, on the 0cm mark, so that the centimeters of the ruler go through the circle of the well and measure the diameter of it. 56. Record this data on your chart and continue to measure each well of each petri dish. 57. After recording your data measurements, restack your petri dishes. 58. At this point you will need to place the four petri dishes into the card board box, make sure to carry the petri dishes by their sides, and do not touch the bottoms, to prevent melting the jello. 59. Do not enclose the petri dishes in the box; leave the flaps of the box down by the outer sides of the box. 60. Leave the petri dishes to sit for 24 hours. 61. After 24 hours, remove the dishes from the box and place them on the flat surface with a blank sheet of printer paper beneath the dishes along with the clear, flat ruler. 62. Remove the control dish off of the stack of petri dishes, and place it on top of the ruler which is on top of the paper. Repeat step 55 and measure the diameter of the new well which should have expanded. Make sure to measure the bottom part of the well, it is okay if the measurements show shrinkage.