Preview

Osmosis: Various Sucrose Concentrations

Good Essays
Open Document
Open Document
617 Words
Grammar
Grammar
Plagiarism
Plagiarism
Writing
Writing
Score
Score
Osmosis: Various Sucrose Concentrations
Abstract: Osmosis is the transport of water across a selectively permeable membrane from a region of a higher water chemical potential to a region of lower water chemical potential. (Tzahi 2006) To demonstrate osmosis lab, artificial cells were made with various sucrose concentrations and were placed into beakers of different sucrose solutions. Overall the results showed that the higher percentage of concentration gradient of sucrose, the more the artificial cells absorbed.
Introduction:
The phenomenon being investigated in the lab was how the concentration gradient affects the rate of osmosis in a cell. Osmosis is the passive movement of water from and area of high concentration, temperature, and pressure. The concentration gradient is the difference in concentration though a solution (Concentration Gradient 2012) They all affect the rate of osmosis by increasing the gradient of the solution, and increasing the energy in the system. For this experiment, it is predicted that an increase in solution concentration will cause a proportional change in the rate of osmosis. Creating artificial cells that contained different concentrations of sucrose tested this hypothesis. In ten-minute increments, the weights of the cells were recorded. Overall a change in weight was found and also the slope to show how much the concentration gradient had on the rate of osmosis.
Materials and Methods: The materials used for the experiment were: five dialysis bags, ten orange clamps to seal our cells, and breakers labeled on thought five. To make sure that the dialysis bags stayed durable they were soaked in water for about ten minutes (or they softened). When the bags were ready to be filled with sucrose solution, the bags were clamped on one side and then opened on the other side. Each bag was then filled with 10 mL of certain solution. Bag one had just tap water, bag two had 20% sucrose, bag three consisted of 40% sucrose, bag four had 60% sucrose, and bag five consisted of

You May Also Find These Documents Helpful

  • Good Essays

    Ap Bio Lab Report Osmosis

    • 850 Words
    • 4 Pages

    of dialysis tubing, 25 mL of sucrose solutions with concentrations of 0.02 Molar, 0.04M, 0.06M, 0.08M, 1.0M, as well as 25mL of distilled water, six 250 mL beakers, a balance, and paper towels. We first cut the dialysis tubing into 6 pieces, each 1ft. long, and placed them into a beaker of water. We then tied off the dialysis tubing and poured 25 mL of distilled water in. We repeated this with the rest of the five pieces dialysis tubing, pouring a different molarity of sucrose solution in different dialysis tubes. After all the tubes were filled and tied, we then dried the bags and weigh each one on the scale. After all the data was recorded, we filled all the beakers about ¾ full of distilled water, placed the bags into each beaker in unison, and waited 30 minutes. Next, the bags were removed from the beakers, dried, and weighed separately. We expected the mass to increase with increasing molarity because with the higher the concentrations, more water would need to be diffused into the bag to reach…

    • 850 Words
    • 4 Pages
    Good Essays
  • Good Essays

    The purpose of this lab was to explore the concept of Osmosis using dialysis tubing and different concentrations of sucrose solutions, and to help one better understand what happens to cells when they are exposed to solutions of differing tonicities.…

    • 977 Words
    • 4 Pages
    Good Essays
  • Better Essays

    Biolab 1208 Lab Report

    • 840 Words
    • 4 Pages

    Introduction: The biological membranes are composed of phospholipid bilayers, each phospholipid with hydrophilic heads and hydrophobic tails, and proteins. This arrangement of the proteins and lipids produces a selectively permeable membrane. Many kinds of molecules surround or are contained within cells, but water is perhaps the single most important molecule in any living system (Hayden and McNeil 2012). Since water molecules are so small, they are constantly going into and out of the cell. Osmosis is a situation where more water molecules are moving across the membrane in one direction than the other (Hayden and McNeil 2012). During osmosis the net movement of water molecules will be from a solution that has a lower osmotic concentration to a solution that has a higher osmotic concentration. When a solution has a higher concentration of solute within the cell than out, it is called hypertonic. When a solution has a lower concentration of solute within the cell than out, it is called hypotonic. And when there are equal concentrations inside and out of the cell, it is called isotonic. The relative osmotic concentration can be determined by a change in mass of the tissue.…

    • 840 Words
    • 4 Pages
    Better Essays
  • Good Essays

    Ap Bio Lab Report

    • 425 Words
    • 2 Pages

    A way to relate this to the real world is that people could use the concept of osmosis to make more accurate administering IVs to put into patients in hospitals. Osmosis is extraordinarily important in the biological processes where the solvent is water. This transport of water and molecules across the membranes is essential to many processes in living organisms and keeping them healthy. In general, this experiment helped understand the different ways osmosis works and how concentration can change the weight of…

    • 425 Words
    • 2 Pages
    Good Essays
  • Satisfactory Essays

    The only increase in mass occurred in the sucrose solution with 0.1 mM (16%). The sucrose solutions from 0.2 mM to 0.5 M had a negative change in mass, with the most being at 0.4 mM at -36%. The trend line (labelled linear series 1) is a negative linear line. Because the percentage change in mass decreased as the sucrose concentration increased, relative osmotic concentration also decreased.…

    • 367 Words
    • 2 Pages
    Satisfactory Essays
  • Better Essays

    Lab 5: Osmosis

    • 1044 Words
    • 5 Pages

    Hypothesis: If we add higher concentrations of sugar to the dialysis tubing, then the net movement of coffee into the dialysis tubing will increase.…

    • 1044 Words
    • 5 Pages
    Better Essays
  • Good Essays

    Ap Bio Lab One

    • 432 Words
    • 2 Pages

    Investigate the relationship between solute concentration and water movement by filling six different dialysis bags with increasing concentrations of sucrose and placing the bags into distilled water. After the time for the experiment has elapsed, compare the initial weight of each bag with its final weight, calculate the percent change in mass, and compare data with rest of class.…

    • 432 Words
    • 2 Pages
    Good Essays
  • Better Essays

    Osmosis Lab Report

    • 1606 Words
    • 7 Pages

    The lab for this paper was conducted for the topic of osmosis, the movement of water from high to low concentration. Five artificial cells were created, each being filled with different concentrated solutions of sucrose. These artificial cells were placed in hypertonic, hypotonic, or isotonic solutions for a period of 90 min. Over time, the rate of osmosis was measured by calculating the weight of each artificial cell on given intervals (every 10 minutes). The resulting weights were recorded and the data was graphed. We then could draw conclusions on the lab.…

    • 1606 Words
    • 7 Pages
    Better Essays
  • Good Essays

    diffusion lab reportt

    • 536 Words
    • 3 Pages

    Abstract dialysis tubing is, made of cellulose because it’s a selectively permeable membrane. In this experiment, we are testing to see if the solution in the beaker moves into the dialysis bag. Which because of that,…

    • 536 Words
    • 3 Pages
    Good Essays
  • Good Essays

    In this experiment, we will investigate the effect of solute concentration on osmosis. A semi‐permeable membrane (dialysis tubing) and sucrose will create an osmotic environment similar to that of a cell. Using different concentrations of sucrose (which is unable to cross the membrane) will allow us to examine the net movement of water across the membrane. In this experiment, we will investigate the effect of solute concentration on osmosis. A semi‐permeable membrane (dialysis tubing) and sucrose will create an osmotic environment similar to that of a cell. Using different concentrations of sucrose…

    • 916 Words
    • 4 Pages
    Good Essays
  • Better Essays

    Lab Report Osmosis

    • 1689 Words
    • 7 Pages

    The main purpose of this paper is to assess the rate of change with osmosis for different concentrations of sucrose in artificial cells. Since the human body is composed of trillions of cells that contain roughly 85% of water, makes osmosis a very important concept (Carmichael, Grabe and Wenger). The forces that affect osmosis are the concentrations of solutes surrounding the cell or inside of the cell. Water will then move across the cell membrane and create a balance of water between the cell and its environment (Reece et al. 133). In order to calculate the average rate of change for our artificial cells, we must understand tonicity as the ability of a nearby solution to cause a cell to lose or gain water, depending on its concentration of non-penetrating solutes relative to solutes inside the cell (Reece et al. 133). The dialysis bags used in this experiment have membranes which are selectively permeable, which only allows particles specifically small enough to pass through (Carmichael, Grabe and Wenger). In a hypotonic solution, water…

    • 1689 Words
    • 7 Pages
    Better Essays
  • Good Essays

    Osmosis Egg Lab Report

    • 1055 Words
    • 5 Pages

    Osmosis is the passing of water or other solutions from an area of high concentration of particles to an area of less concentration. The cell membrane is an organelle, selectively permeable, and only allows certain materials to pass through; it can allow smaller molecules to pass while blocking larger molecules. Molecules that are blocked can be moved through active transport, such as, through proteins that are embedded in the bilayer of lipids.…

    • 1055 Words
    • 5 Pages
    Good Essays
  • Good Essays

    vugj

    • 460 Words
    • 2 Pages

    Introduction: Osmosis is the diffusion of water from a higher concentration to a lower one through a semi-permeable membrane and occurs in the cells of organisms. It is affected by the addition of solute which would lower the water potential, making water potential and solute concentration inversely related. The concept of osmosis loosely described is that the presence of more solute outside the cell means the presence of less solvent (or water molecules in this case) and vice versa; the goal of osmosis is for the water molecules inside and outside of the cell to be equal, causing equilibrium and a stop to net water movement. The goal for a recent lab was to test whether cubes of potato would gain or lose weight depending on the amount of molar concentration of sucrose they soaked in. Solutions made of different molar concentrations of the solute sucrose were prepared before this lab. Potato cubes were weighed and recorded as the initial mass in the data. Four cubes of potato were placed into 100 mL of each solution and let stand overnight. The cubes were then taken out for the final total mass to be measured and recorded. In this experiment, the potato cubes were the dependent variables, and the molar concentrations were the independent or manipulated variables.…

    • 460 Words
    • 2 Pages
    Good Essays
  • Better Essays

    The purpose of this lab was to observe the rate of osmosis and diffusion, as well as the effect of molecular size of the particles on this rate. Part I of the lab was a demonstration of osmosis and diffusion, that dealt with raisins in different liquid environments, each with a different concentration of sugar. Part IV of the lab was using the same idea as the demonstration, by putting objects in different concentrations of a substance; in this case elodea leaves in salt water. In both cases, the objects in a greater concentration of the substance were stripped of their water. However, where there was a little or no concentration of sugar or salt, the objects did not lose their water, and in the case of the raisin, became saturated with excess…

    • 2315 Words
    • 10 Pages
    Better Essays
  • Good Essays

    Osmosis Lab Report

    • 2530 Words
    • 11 Pages

    INTRODUCTION: In order to fully understand the concept of this experiment, one must first be introduced to diffusion. Diffusion is a spontaneous process by which molecule particles move from one area that is highly concentrated to another area in which its concentration is lower. Cells contain fluids and are surrounded by fluids; in order for a cell to function it is required to be in a balanced state. The progress in which a cell is in its balanced state is called equilibrium. Diffusion is a functioning way for cells to reach their equilibrium. Equilibrium is reached by controlling what enters and exits the cell through a cell membrane that selectively filters molecules by slowing down their movement, allowing them to pass through, or not allowing them to pass through the membrane. Diffusion and osmosis go hand-in-hand. Osmosis is generally the same as diffusion, however, deals solely with water. Osmotic pressure is the pressure of a solution against a semi-permeable membrane to prevent water from flowing into the membrane. In this lab, we are going to study tonicity; tonicity is the measure of this osmotic pressure and is the differential of pressure between two solutions separated by a selective membrane. To help identify the relative concentrations of solute particles of different solutions, we must understand that there are three possible differences in concentrations between a cell and its environment. The terms hypotonic, hypertonic, and isotonic are used in referring to the identification of the possible relative concentrations. The first term, hypotonic, is the solution that contains lower concentrations of solute particles, which means that the concentration inside the cell is greater than the concentration outside. A hypotonic solution causes the cell to swell in size. The second term, hypertonic, is the solution that contains higher concentration of solute…

    • 2530 Words
    • 11 Pages
    Good Essays