Pglo Transformation
Miguel Felix pGLO Transformation
Mr. Betz
AP Biology
14 December 2012
Abstract
The purpose of this experiment is to determine the effects of the addition of a plasmid to a bacterial cell. The bacteria E. Coli was separated into two groups: one where the pGLO plasmid was added to the bacteria, which contains the genes of fluorescence and resistance to antibiotics, and the other lacking the plasmid. The two groups then placed in agar plates simulating different environments: the bacteria lacking the pGLO plasmid was subjected to an environment solely contaiing nutrients and another containing nutrients and ampicillin; the bacteria containing pGLO was subjected to an environment containing solely nutrients and one containing nutrients, ampicillin, and the sugar arabinose. After being allowed to grow in their respective environments, the following agar plates grew E. Coli colonies: agar containing nutrients and a colony without the pGLO plasmid, agar plate containing nutrients, ampicillin, and bacteria containing the pGLO plasmid, and agar containing nutrients, ampicillin, arabinose, and bacteria containing the pGLO gene; while the agar plate containing both ampicillin and the bacteria not being exposed to the pGLO plasmid died off.
Introduction
By allowing the bacteria E. Coli to be exposed to the pGLO bacteria, both the evolutionary methods of bacteria and the applications of bioengineering. By allowing bacteria to uptake genes from another organism, a jellyfish in this case, through the use of a plasmid, the ability of bacteria to produce possibly beneficial bacteria could be tested. My hypothesis is that both samples of the bacteria containing the pGLO plasmid shall express fluorescence but the one placed in the agar plate containing arabinose shall express this trait more vibrantly. Also, the bacteria without the plasmid will not glow and the colony placed in the agar plate containing ampicillin will die off completely. The survivability of the
Mr. Betz
AP Biology
14 December 2012
Abstract
The purpose of this experiment is to determine the effects of the addition of a plasmid to a bacterial cell. The bacteria E. Coli was separated into two groups: one where the pGLO plasmid was added to the bacteria, which contains the genes of fluorescence and resistance to antibiotics, and the other lacking the plasmid. The two groups then placed in agar plates simulating different environments: the bacteria lacking the pGLO plasmid was subjected to an environment solely contaiing nutrients and another containing nutrients and ampicillin; the bacteria containing pGLO was subjected to an environment containing solely nutrients and one containing nutrients, ampicillin, and the sugar arabinose. After being allowed to grow in their respective environments, the following agar plates grew E. Coli colonies: agar containing nutrients and a colony without the pGLO plasmid, agar plate containing nutrients, ampicillin, and bacteria containing the pGLO plasmid, and agar containing nutrients, ampicillin, arabinose, and bacteria containing the pGLO gene; while the agar plate containing both ampicillin and the bacteria not being exposed to the pGLO plasmid died off.
Introduction
By allowing the bacteria E. Coli to be exposed to the pGLO bacteria, both the evolutionary methods of bacteria and the applications of bioengineering. By allowing bacteria to uptake genes from another organism, a jellyfish in this case, through the use of a plasmid, the ability of bacteria to produce possibly beneficial bacteria could be tested. My hypothesis is that both samples of the bacteria containing the pGLO plasmid shall express fluorescence but the one placed in the agar plate containing arabinose shall express this trait more vibrantly. Also, the bacteria without the plasmid will not glow and the colony placed in the agar plate containing ampicillin will die off completely. The survivability of the