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Pglo Transformation Lab Report

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Pglo Transformation Lab Report
The purpose of this experiment was understand the process of transformation and the effects on gene expression.
The pGLO(-) culture had growth on the LB medium, while the LB amp and LB amp + ara mediums had no growth. It was expected that the LB medium had growth on the plate because it served as a control. The LB amp and LB amp + ara had no growth or glow under UV light because they were not successfully transformed and still contained the antibiotic ampicillin that prevented the growth of E. coli (3).
The pGLO(+) culture had growth on all three plates. The LB medium was expected to have growth on it because it also served as a control. The LB amp plate had growth on it because the culture was antibiotic resistant due to the pGLO transformation
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