Pglo Transformation Lab Report

Transformation in bacteria is the genotype alteration by the uptake of naked, foreign DNA from the environment. This concept of transformation was first discovered when Fred Griffith an experiment using mice and strains of pneumonia. Griffith concluded that a “principle” was transferred from heat-killed S strains to the R strains, which transformed them into deadly S strains. Oswald Avery later determined, through a series of experiments, that DNA was the “principle” that caused the R stains to become S strains that killed the mice. Bacteria will typically go through transformation when put under stress. In this experiment, the bacteria will be treated with calcium chloride to make them competent. As the chloride ions enter the cell, water