Rainbow Transformation1 Lab Report Sample

For the completion of this experiment the procedures were guided with the Rainbow Transformation1 lab manual. An Escherichia coli bacterial reference plate was used to obtain colonies which were resuspended into a CaCl2 solution that was previously kept on an ice bath. The rainbow transformation mixture containing the plasmid DNA was then added to half of the E. coli cells. These cells were later placed into a water bath set to 42ºC and “heat shocked” to promote the entrance of DNA into the cells. Moreover, a Recovery Broth was added to the sample and the sample was left undisturbed for 30 minutes at room temperature so the “heat shocked” bacteria would acquire the antibiotic resistance gene.
After the elapsed time, the samples were transferred