Pglo Transformation Lab Report
Malak Zomrawi
4/9/15
Bacterial Transformation
I. Abstract
In the lab, the purpose is to see if we could move genes using plasmid. As well as getting better understand of transformation methods using shock wave. To see the effects five trays are being used containing LB nutrient broth. The results showed that the LB, ampicillin, and arabinose with a positive pGLO had the most amount of growth compared to the other four trays. Although when there is arabinose there is no fluorescence, fluorescence was seen under UV lighting. What will further be discussed are the errors, future possibilities and how to change the outcomes and also ethical implications.
II. Introduction:
Genetic transformation to E.coli is a very rare entity because only one in a million …show more content…
Because of the pressure from when a pipet is squeezed to hard we could not get the amount required. Because of this the experiment could not 100 percent described correctly. If one colony was taken, the odds of two colonies being the same is very low. Many future implications can be done with this experiment. It does not have to fully be limited to bacteria. This lab can lead to organisms’ modification such as plants. Plants can take in plasmid to gain new traits. Which can lead to speed in growth rate, new feature or be used as a resistance for any harm the plant may face.
Overall the experiment turned out to be a success for the most part. We were able to see some of the colonies to appear under the UV light. My predictions of what I expected to show growth ended up not showing growth at all and the one, I predicted to show no growth ended up showing the most growth out of all of them. I predicted that the LB/Amp/Ara with +pGLO would show the no growth because it had arabinose, but it later ended up having the most growth amongst the other trays. The others that had growth were very small and hard to
4/9/15
Bacterial Transformation
I. Abstract
In the lab, the purpose is to see if we could move genes using plasmid. As well as getting better understand of transformation methods using shock wave. To see the effects five trays are being used containing LB nutrient broth. The results showed that the LB, ampicillin, and arabinose with a positive pGLO had the most amount of growth compared to the other four trays. Although when there is arabinose there is no fluorescence, fluorescence was seen under UV lighting. What will further be discussed are the errors, future possibilities and how to change the outcomes and also ethical implications.
II. Introduction:
Genetic transformation to E.coli is a very rare entity because only one in a million …show more content…
Because of the pressure from when a pipet is squeezed to hard we could not get the amount required. Because of this the experiment could not 100 percent described correctly. If one colony was taken, the odds of two colonies being the same is very low. Many future implications can be done with this experiment. It does not have to fully be limited to bacteria. This lab can lead to organisms’ modification such as plants. Plants can take in plasmid to gain new traits. Which can lead to speed in growth rate, new feature or be used as a resistance for any harm the plant may face.
Overall the experiment turned out to be a success for the most part. We were able to see some of the colonies to appear under the UV light. My predictions of what I expected to show growth ended up not showing growth at all and the one, I predicted to show no growth ended up showing the most growth out of all of them. I predicted that the LB/Amp/Ara with +pGLO would show the no growth because it had arabinose, but it later ended up having the most growth amongst the other trays. The others that had growth were very small and hard to