Potato Enzyme Lab Report
The Effect of pH on Enzyme Activity
A piece of Solanum tuberosum (potato) was removed and mixed with distilled water in a blender. The resulting solution was filtered through multiple layers of cheese cloth to filter out the liquid by eliminating any large pieces in the solution. The solution created was catechol. Five different solutions were prepared as blanks with each test tube containing 6.0mL of a different pH (pH 4, pH6, pH7, pH8, pH10) of phosphate buffer, 1.0mL of the enzyme and 1.0mL of water. Five more solutions were prepared in the same fashion except without the 1.0mL of water. These five experimental solutions were capped and mixed by inversion. After mixing, 1.0mL of catechol was added to each of the five experimental solutions and they were each inverted every minute for five minutes. The spectrophotometer was calibrated using the blank solution. The experimental solution, with the same pH as the blank, was …show more content…
Five more solutions were prepared in the same fashion except with 1.0mL of catechol instead of 1.0mL of water. These experimental solutions were covered and mixed. Each solution and its sister blank (blank to be tested at the same temperature) were placed in one of the temperatures, with the 20°C solution left at room temperature. The solutions were left for 5 minutes in their respective temperatures to allow the buffers to reach the selected temperature. After 5 minutes, the solutions were taken out and 1.0mL of enzyme was added to each solution. The solutions were mixed and returned to their respective temperatures. The solutions were mixed at one minute intervals for 5 minutes. The spectrophotometer was calibrated using the blank solution. After calibration, the sister experimental solution was tested for its absorption. All of the solutions were tested at their value closest to
A piece of Solanum tuberosum (potato) was removed and mixed with distilled water in a blender. The resulting solution was filtered through multiple layers of cheese cloth to filter out the liquid by eliminating any large pieces in the solution. The solution created was catechol. Five different solutions were prepared as blanks with each test tube containing 6.0mL of a different pH (pH 4, pH6, pH7, pH8, pH10) of phosphate buffer, 1.0mL of the enzyme and 1.0mL of water. Five more solutions were prepared in the same fashion except without the 1.0mL of water. These five experimental solutions were capped and mixed by inversion. After mixing, 1.0mL of catechol was added to each of the five experimental solutions and they were each inverted every minute for five minutes. The spectrophotometer was calibrated using the blank solution. The experimental solution, with the same pH as the blank, was …show more content…
Five more solutions were prepared in the same fashion except with 1.0mL of catechol instead of 1.0mL of water. These experimental solutions were covered and mixed. Each solution and its sister blank (blank to be tested at the same temperature) were placed in one of the temperatures, with the 20°C solution left at room temperature. The solutions were left for 5 minutes in their respective temperatures to allow the buffers to reach the selected temperature. After 5 minutes, the solutions were taken out and 1.0mL of enzyme was added to each solution. The solutions were mixed and returned to their respective temperatures. The solutions were mixed at one minute intervals for 5 minutes. The spectrophotometer was calibrated using the blank solution. After calibration, the sister experimental solution was tested for its absorption. All of the solutions were tested at their value closest to