Potato Osmosis Lab Report
Abstract The experiment was carried out to investigate at which point the potato has reached its isotonic environment. From the results, when the concentration increase to 0.3M, the mass of the potato slices decreased by -3.26%. We can conclude we see that as we increased the sucrose concentration it was a decrease in the percent change.
Introduction
All cells have membranes that are selectively permeable. In other words, they allow certain things in and certain substances are not allowed to enter the inside of the cell. Osmosis is the process of water movement across the membranes from an area of high water concentration to an area of low water concentration to reach a sense of equilibrium. Diffusion is what enables osmosis to occur. …show more content…
This is the process of movement of particles from an area of higher concentration to an area of lower concentration. Hypotonic, hypertonic, and isotonic are the three terms that we use to describe a solute concentrations relatively to another solute concentration. The tonicity depends on the level of solutes within the cell and the environment; hypotonic solutions contains a low concentration of solute relative to a cell which diffuses water into a cell, hypertonic solutions contains a high concentration of solute relative to a cell which diffuses water out of a cell, and an isotonic solution has same amount of concentration of solute which neither diffuses water in or out the cell. The experiment was carried out to investigate at which point the potato has reached its isotonic environment. We prepared six different sucrose concentration (0.0M, 0.1M, 0.2M, 0.3M, 0.4M, 0.5M) conditions to be tested and cut thin slices of potatoes. We weight each section of potatoes before places them in there sucrose concentration. We recorded the final mass, then using the formula, % change in mass = final mass (g) – initial mass (g) x100, Initial mass we calculate the percent change in mass for each sucrose concentrations. This experiment was important to explore because this could determine the best method to keep the potatoes preserved during harvesting time when making deliveries from farmers to the markets.
Null hypothesis: If I change the sucrose concentration, then the % change in mass of the potato will not change.
Alternative hypothesis: If I change the sucrose concentration, then the % change in mass of the potato will change.
Methods and Materials
Balance and weigh boat
Marking pen
6 potato tuber segments
Razor blade
Forceps
Paper towels
Screen
Metric ruler
Cold tap water
Disposable cups
100 mL deionized water
100 mL 0.1 M sucrose solution
100 mL 0.2 M sucrose solution
100 mL 0.3 M sucrose solution
100 mL 0.4 M sucrose solution
100 mL 0.5 M sucrose solution
Six samples of potato tubers were obtained.
They were each cut in small, thin segments with a razor blade and then rinsed. Each sample was then weighed with a balance to get the initial mass recorded.
Six disposable cups were labeled and filled with one of the 100 mL sucrose solutions (0.0M, 0.1M, 0.2M, 0.3M, 0.4M and 0.5M). One sample of potato segments was added to each of the sucrose solutions in the disposable cups. The potatoes were left to soak for one hour.
After the one hour was up, the potatoes were removed and blotted with a paper towels. Finally they were weighed again for the final mass to be recorded and from there the percent change in mass was calculated for each sample.
Results
Figure 1: As the sucrose concentration increased, the % change in the mass decreased. As you look to 0.0M sucrose concentration, the mass of the potato slices increased by 9.02%. As the sucrose concentration increased to 0.1M, the mass of the potatoes increased by 8.77%. As the sucrose concentration increased to 0.2M, the mass of the potatoes increased by 4.12%. When the concentration increase to 0.3M, the mass of the potato slices decreased by -3.26%. At 0.4M sucrose concentration, the mass of the potato slices decreased significantly by 29.22%. At 0.5M sucrose …show more content…
concentration, the mass of the potato slices decreased by 16.51%.
Conclusion The experiment outlined that we had to let the potatoes sit in the sucrose concentration for 60 minutes, but time in class restricted us instead the potatoes sat in the concentration for 35
minutes.
When you look from 0.0M to 0.1M sucrose concentration, the percent change in mass decreased from 9.02% to 8.77% which had no significant difference between these points. From concentration 0.1M to 0.2M there was a decrease by 4.02% and a significant difference between the two points. These three sucrose concentrations are found to be in a hypnotic solution where water is diffused in the cell which the potato is gaining mass. Now looking at sucrose concentrations 0.2M to 0.3M, the percent change in mass decrease from 4.02% to -3.26%. This causes to have a significant difference between the two points. We increase from 0.3M to 0.4M we have a dramatically decrease from -3.26% to -29.22%. You can conclude from these points that there is a significant difference. As we look at 0.4M to 0.5M we have an increase in a percent change in mass from -29.22% to -16.51%. These three sucrose concentrations are found to be in a hypertonic solution where water is diffused out the cell which the potato is losing mass. At the sucrose concentrations 0.2M and 0.3M we reached the point where the potato is at an isotonic environment, it would be between 4.02% to
-3.26%. Our results lead us to have significant difference.. The sucrose concentrations that are in a hypotonic and hypertonic solutions support the alternative hypothesis. The sucrose concentration of 0.0M, 0.1M, and 0.2M were all in a hypotonic solution to the potatoes slices. The sucrose concentrations of 0.3M, 0.4M, and 0.5 are all in a hypertonic solution. Between the sucrose concentrations of 0.2M and 0.3M, there is a point of equilibrium where the solution is isotonic to the potato
Introduction
All cells have membranes that are selectively permeable. In other words, they allow certain things in and certain substances are not allowed to enter the inside of the cell. Osmosis is the process of water movement across the membranes from an area of high water concentration to an area of low water concentration to reach a sense of equilibrium. Diffusion is what enables osmosis to occur. …show more content…
This is the process of movement of particles from an area of higher concentration to an area of lower concentration. Hypotonic, hypertonic, and isotonic are the three terms that we use to describe a solute concentrations relatively to another solute concentration. The tonicity depends on the level of solutes within the cell and the environment; hypotonic solutions contains a low concentration of solute relative to a cell which diffuses water into a cell, hypertonic solutions contains a high concentration of solute relative to a cell which diffuses water out of a cell, and an isotonic solution has same amount of concentration of solute which neither diffuses water in or out the cell. The experiment was carried out to investigate at which point the potato has reached its isotonic environment. We prepared six different sucrose concentration (0.0M, 0.1M, 0.2M, 0.3M, 0.4M, 0.5M) conditions to be tested and cut thin slices of potatoes. We weight each section of potatoes before places them in there sucrose concentration. We recorded the final mass, then using the formula, % change in mass = final mass (g) – initial mass (g) x100, Initial mass we calculate the percent change in mass for each sucrose concentrations. This experiment was important to explore because this could determine the best method to keep the potatoes preserved during harvesting time when making deliveries from farmers to the markets.
Null hypothesis: If I change the sucrose concentration, then the % change in mass of the potato will not change.
Alternative hypothesis: If I change the sucrose concentration, then the % change in mass of the potato will change.
Methods and Materials
Balance and weigh boat
Marking pen
6 potato tuber segments
Razor blade
Forceps
Paper towels
Screen
Metric ruler
Cold tap water
Disposable cups
100 mL deionized water
100 mL 0.1 M sucrose solution
100 mL 0.2 M sucrose solution
100 mL 0.3 M sucrose solution
100 mL 0.4 M sucrose solution
100 mL 0.5 M sucrose solution
Six samples of potato tubers were obtained.
They were each cut in small, thin segments with a razor blade and then rinsed. Each sample was then weighed with a balance to get the initial mass recorded.
Six disposable cups were labeled and filled with one of the 100 mL sucrose solutions (0.0M, 0.1M, 0.2M, 0.3M, 0.4M and 0.5M). One sample of potato segments was added to each of the sucrose solutions in the disposable cups. The potatoes were left to soak for one hour.
After the one hour was up, the potatoes were removed and blotted with a paper towels. Finally they were weighed again for the final mass to be recorded and from there the percent change in mass was calculated for each sample.
Results
Figure 1: As the sucrose concentration increased, the % change in the mass decreased. As you look to 0.0M sucrose concentration, the mass of the potato slices increased by 9.02%. As the sucrose concentration increased to 0.1M, the mass of the potatoes increased by 8.77%. As the sucrose concentration increased to 0.2M, the mass of the potatoes increased by 4.12%. When the concentration increase to 0.3M, the mass of the potato slices decreased by -3.26%. At 0.4M sucrose concentration, the mass of the potato slices decreased significantly by 29.22%. At 0.5M sucrose …show more content…
concentration, the mass of the potato slices decreased by 16.51%.
Conclusion The experiment outlined that we had to let the potatoes sit in the sucrose concentration for 60 minutes, but time in class restricted us instead the potatoes sat in the concentration for 35
minutes.
When you look from 0.0M to 0.1M sucrose concentration, the percent change in mass decreased from 9.02% to 8.77% which had no significant difference between these points. From concentration 0.1M to 0.2M there was a decrease by 4.02% and a significant difference between the two points. These three sucrose concentrations are found to be in a hypnotic solution where water is diffused in the cell which the potato is gaining mass. Now looking at sucrose concentrations 0.2M to 0.3M, the percent change in mass decrease from 4.02% to -3.26%. This causes to have a significant difference between the two points. We increase from 0.3M to 0.4M we have a dramatically decrease from -3.26% to -29.22%. You can conclude from these points that there is a significant difference. As we look at 0.4M to 0.5M we have an increase in a percent change in mass from -29.22% to -16.51%. These three sucrose concentrations are found to be in a hypertonic solution where water is diffused out the cell which the potato is losing mass. At the sucrose concentrations 0.2M and 0.3M we reached the point where the potato is at an isotonic environment, it would be between 4.02% to
-3.26%. Our results lead us to have significant difference.. The sucrose concentrations that are in a hypotonic and hypertonic solutions support the alternative hypothesis. The sucrose concentration of 0.0M, 0.1M, and 0.2M were all in a hypotonic solution to the potatoes slices. The sucrose concentrations of 0.3M, 0.4M, and 0.5 are all in a hypertonic solution. Between the sucrose concentrations of 0.2M and 0.3M, there is a point of equilibrium where the solution is isotonic to the potato