Protein Purification Lab Report
To purify the protein in the cell lysate from lab 1 through nickel affinity chromatography. Protein purification should result in only one type of protein ideally, which is the protein of interest, wt-DHFR and mut-DHFR in this case. A pure protein allows for further analysis on the protein to be conducted, such as its concentration (Bradford assay), its molecular weight, and its biological activity.
2. Overview of experiments
Buffer Preparation
Add the liquid sodium phosphate, solid sodium chloride and liquid imidazole in proper amount and concentration for three different buffers.
Save 100µL of each cell lysate into two tubes for lab 3 and 4.
DHFR Protein Purification
Keep every reagent on ice. Label all 12 collection tubes. Remove the
2. Overview of experiments
Buffer Preparation
Add the liquid sodium phosphate, solid sodium chloride and liquid imidazole in proper amount and concentration for three different buffers.
Save 100µL of each cell lysate into two tubes for lab 3 and 4.
DHFR Protein Purification
Keep every reagent on ice. Label all 12 collection tubes. Remove the