Quantitative Glucose Test
LAB 2:
DATE: 29TH September, 2011.
FORM CLASS: L6 3
SUBJECT: Biology
TITLE: Quantitative Glucose Test
AIM: To determine the amount of glucose in three unknown samples namely A, B and C
INTRODUCTION:
Biological molecules are held together by covalent bonds, hydrogen bonds among others bonds in various ways to produce large molecules called macromolecules. Simple organic compounds and macromolecules molecules vary in structure and can be distinguished by their functional groups. Molecules of a certain class have similar chemical properties because they have the same functional group. A chemical test that is sensitive to that group can be used to identify molecules that are in that class. There are also tests which measure the quantity of the particular biological molecule present in the substance. This was done so that one can be mindful of the quantities of certain biological molecules entering the body. This awareness can prevent and control diseases such as diabetes mellitus which is a condition where blood sugar level is not controlled correctly and affected people take insulin to help to control their glucose levels and test their blood to determine the level of glucose in it. There are a variety of different ways in which blood glucose level can be measured.
It is often important to measure the concentration of glucose in a solution. In this experiment a variety of solutions will be tested for the glucose concentration of known value and a graph, drawn to show the results. This type of graph is known as a Standard Curve. This graph will then be used to estimate the glucose concentration in an unknown solution. This is the method which was used in hospital labs to measure the glucose level in blood samples. Glucose (C6H12O6) is a monosaccharide reducing sugar. In this reaction the glucose readily donates electrons which are accepted by the permanganate causing it to change colour. The time taken for the pink colour of the potassium permanganate to disappear
DATE: 29TH September, 2011.
FORM CLASS: L6 3
SUBJECT: Biology
TITLE: Quantitative Glucose Test
AIM: To determine the amount of glucose in three unknown samples namely A, B and C
INTRODUCTION:
Biological molecules are held together by covalent bonds, hydrogen bonds among others bonds in various ways to produce large molecules called macromolecules. Simple organic compounds and macromolecules molecules vary in structure and can be distinguished by their functional groups. Molecules of a certain class have similar chemical properties because they have the same functional group. A chemical test that is sensitive to that group can be used to identify molecules that are in that class. There are also tests which measure the quantity of the particular biological molecule present in the substance. This was done so that one can be mindful of the quantities of certain biological molecules entering the body. This awareness can prevent and control diseases such as diabetes mellitus which is a condition where blood sugar level is not controlled correctly and affected people take insulin to help to control their glucose levels and test their blood to determine the level of glucose in it. There are a variety of different ways in which blood glucose level can be measured.
It is often important to measure the concentration of glucose in a solution. In this experiment a variety of solutions will be tested for the glucose concentration of known value and a graph, drawn to show the results. This type of graph is known as a Standard Curve. This graph will then be used to estimate the glucose concentration in an unknown solution. This is the method which was used in hospital labs to measure the glucose level in blood samples. Glucose (C6H12O6) is a monosaccharide reducing sugar. In this reaction the glucose readily donates electrons which are accepted by the permanganate causing it to change colour. The time taken for the pink colour of the potassium permanganate to disappear