The initial room temperature should be calculated using a thermometer and a marker is used to name beakers 1 and 2. Divide the 10 test tubes into two groups and name them 1-5 in both groups. 5 different stopwatches are used to record the time for the disappearance of the blue-black color in test tubes, name them 1-5 also. Stopwatch 1 corresponds to test tube 1 in beaker 1 i.e. 0°C and the room temperature (that should be around 20-25°C), and stopwatch 5 corresponds to test tube 5 in a water bath of 80°C. Record the room temperature. …show more content…
Beaker two is only filled with tap water. 3 Put a thermometer in both beakers and after one or two minutes, see if they have reached 0°C and room temperature. Once the beakers have reached the desired temperatures (0°C and room temperatures), the experiment may start.
The amylase and starch solutions should be checked in order to test their pH values and be sure they are the precise and correct pH needed for the experiment. If desired, the pH values can be measured using pH strips. Add around 2cm3 of amylase solution into a 10cm3 measuring cylinder. After precisely reaching 2cm3 of amylase, the solution is to be poured into test tubes 1-5 of group 1. 2cm3 of starch solution should be poured in test tubes 1-5 of group 2.
Test tubes 1 from both groups are to be put into beaker 1 and test tubes labelled 2 should be put into beaker to. Place test tubes 3 in the test tube rack and within a 40°C water bath. Place test tubes 4 and 5 in test tube racks in a 60°C and 80°C water