Saponification Value of Oil
Fat and Oil
Saponification value of Fat and Oil
Acid⋅base titration (non aqueous) by Automatic Potentiometric Titrator
Standard
JIS ASTM ISO K 0070 D 5558 3657 Standard Test Method for Fat and Oil
1.Abstract
Saponification value is expressed by potassium hydroxide in mg required to saponify one (1) gram of fat. It depends on the kind of fatty acid contained in the fat. Measurement of saponification value is performed according to the below listed official test methods. Here we test a sample of fatty acid. The sample is first saponified by adding 0.5mol/L potassium hydroxide ethanol, and then the excessive potassium hydroxide is titrated with 0.5mol/L hydrochloric acid until the endpoint is reached. EP is determined by the maximum inflexion point on titration curve. R COOH + KOH KOH + HCl → → R COOK + H2O KCl + H2O
2.Reference
1) JIS K 0070-1992 Test Method for Acidity, Saponification value, Ester value, Iodine value and Hydroxy value of Chemical products and Unsaponifiables 2) ASTM D 5558-95 Standard Test Method for Determination of the Saponification Value of Fats and Oils 3) ISO 3657 : 2002 Animal and vegetable fats and oils – Determination of saponification value 4) Method （I） 3.3.2-1996 Saponification Value from Standard Test Method for Fat and Oil by Japanese Oil Chemistry Society
3.Cautions in measurement
1) When the sample is heated in flask for saponification, gently heat it so that backflow ethanol will not reach the top of cooling pipe fitted to the flask. 2) Replace junction liquid of reference electrode with saturated sodium perchlorate acetic acid.
TIB-99307 Ver.01 1/4
4.Post-measurement care
After the electrode is rinsed with water, keep the tip of electrode dipped in pure water for storage.
5.Test equipment
Main unit：Automatic potentiometric titrator （Standard preamplifier STD－） Electrode： Standard Combination glass electrode （Change junction liquid to saturated sodium perchlorate acetic acid）
6.Reagent
Reagent Reagent ： ： 0.5mol/L
Saponification value of Fat and Oil
Acid⋅base titration (non aqueous) by Automatic Potentiometric Titrator
Standard
JIS ASTM ISO K 0070 D 5558 3657 Standard Test Method for Fat and Oil
1.Abstract
Saponification value is expressed by potassium hydroxide in mg required to saponify one (1) gram of fat. It depends on the kind of fatty acid contained in the fat. Measurement of saponification value is performed according to the below listed official test methods. Here we test a sample of fatty acid. The sample is first saponified by adding 0.5mol/L potassium hydroxide ethanol, and then the excessive potassium hydroxide is titrated with 0.5mol/L hydrochloric acid until the endpoint is reached. EP is determined by the maximum inflexion point on titration curve. R COOH + KOH KOH + HCl → → R COOK + H2O KCl + H2O
2.Reference
1) JIS K 0070-1992 Test Method for Acidity, Saponification value, Ester value, Iodine value and Hydroxy value of Chemical products and Unsaponifiables 2) ASTM D 5558-95 Standard Test Method for Determination of the Saponification Value of Fats and Oils 3) ISO 3657 : 2002 Animal and vegetable fats and oils – Determination of saponification value 4) Method （I） 3.3.2-1996 Saponification Value from Standard Test Method for Fat and Oil by Japanese Oil Chemistry Society
3.Cautions in measurement
1) When the sample is heated in flask for saponification, gently heat it so that backflow ethanol will not reach the top of cooling pipe fitted to the flask. 2) Replace junction liquid of reference electrode with saturated sodium perchlorate acetic acid.
TIB-99307 Ver.01 1/4
4.Post-measurement care
After the electrode is rinsed with water, keep the tip of electrode dipped in pure water for storage.
5.Test equipment
Main unit：Automatic potentiometric titrator （Standard preamplifier STD－） Electrode： Standard Combination glass electrode （Change junction liquid to saturated sodium perchlorate acetic acid）
6.Reagent
Reagent Reagent ： ： 0.5mol/L