spectrophotometric analysis of copper sulphate
spectrophotometric analysis of copper sulphate
Introduction: In the modern usage. The term spectroscopy includes a large group of techniques which differ widely in their mode of application and information they reveal. As a useful working definition, spectroscopy is a study of interaction of electromagnetic radiation with matter in the experiment it means it's the usage of ultra-light to measure the absorbance of the copper sulpate. The regions of the electromagnetic spectrum immediately adjacent to the high- and low- energy extremes of the visible region are known as the ultraviolet (UV) and infrared (IR) regions respectively. Thus a quantum of UV radiation possesses more energy than one in the visible region whilst the reverse is true for quantum of IR radiation. All spectrophotometric methods depends on the application of two basic laws which, combined together, are kwon as the Beer-Lambert law.
The equation wdemonstrate that the amount of light absorbed is proportional to the number of molecules of the chromosphere, through which the light pass.
Aims: The aim of the experiment is to calculate the concentration of copper sulpate which gives the absorbance for each concentration and learn the techniques of using the spectrophotometry to detect the absorbance at the specific wavelength by the light . In addition, to perform dilutions and relate concentrations obtained for diluted materials to the concentrations of the undiluted products also understand Beer Lambert law and it's relationship to solution concentration.
Methods: preparing a spectrometer which contained a standard solution of copper sulphate ( ) and diluted with the distilled water in the cuvette
Results:
Table (1) sample volume of copper sulpate
(100 mmol L^-1) standard solution / mL
Volume of water
/ mL copper sulpate
/ mmol L^-1 measured absorbance
A807
blank
0
10
0
0
2
2
8
20
0.246
3
4
6
40
0.48
4
5
5
50
0.591
5
6
4
60
0.721
6
8
2
80
Introduction: In the modern usage. The term spectroscopy includes a large group of techniques which differ widely in their mode of application and information they reveal. As a useful working definition, spectroscopy is a study of interaction of electromagnetic radiation with matter in the experiment it means it's the usage of ultra-light to measure the absorbance of the copper sulpate. The regions of the electromagnetic spectrum immediately adjacent to the high- and low- energy extremes of the visible region are known as the ultraviolet (UV) and infrared (IR) regions respectively. Thus a quantum of UV radiation possesses more energy than one in the visible region whilst the reverse is true for quantum of IR radiation. All spectrophotometric methods depends on the application of two basic laws which, combined together, are kwon as the Beer-Lambert law.
The equation wdemonstrate that the amount of light absorbed is proportional to the number of molecules of the chromosphere, through which the light pass.
Aims: The aim of the experiment is to calculate the concentration of copper sulpate which gives the absorbance for each concentration and learn the techniques of using the spectrophotometry to detect the absorbance at the specific wavelength by the light . In addition, to perform dilutions and relate concentrations obtained for diluted materials to the concentrations of the undiluted products also understand Beer Lambert law and it's relationship to solution concentration.
Methods: preparing a spectrometer which contained a standard solution of copper sulphate ( ) and diluted with the distilled water in the cuvette
Results:
Table (1) sample volume of copper sulpate
(100 mmol L^-1) standard solution / mL
Volume of water
/ mL copper sulpate
/ mmol L^-1 measured absorbance
A807
blank
0
10
0
0
2
2
8
20
0.246
3
4
6
40
0.48
4
5
5
50
0.591
5
6
4
60
0.721
6
8
2
80