Preview

Streak a Bacterial Culture Anticeptics

Powerful Essays
Open Document
Open Document
1781 Words
Grammar
Grammar
Plagiarism
Plagiarism
Writing
Writing
Score
Score
Streak a Bacterial Culture Anticeptics
Introduction
The working hypothesis being tested is if any of the three products; Scope Mouthwash (Scope), LISTERINE® Antiseptic Mouthwash (Listerine), Bactine Original First Aid Liquid (Bactine), and Povidone-Iodine Solution, 10% Topical Microbicide Antiseptic (Iodine) are an appropriate antiseptic to can kill a sample bacterial contaminant found in the local environment, and if so, which is the most effective?
The purpose of this experiment is to determine the antibacterial effectiveness of Scope, Listerine, Bactine, and Iodine in relation to each other.
Scope, Listerine, Bactine, and Iodine each are believed to be effective antiseptics: * Scope Mouthwash - Kills 99% of bad breath germs (in lab tests) http://www.scopemouthwash.com/scope-products/scope-mouthwash-original-mint.aspx * LISTERINE® Antiseptic Mouthwash - This effective rinse kills germs in virtually 100% of the mouth, penetrating to kill plaque biofilm. http://www.jjdentalprofessional.com/deepestclean * Bactine Original First Aid Liquid has provided soothing infection protection for over 50 years. http://www.bactine.com/original.htm * Iodine - A commonly used antimicrobial agent is Povidone-iodine (Betadine), a complex of iodine, the bactericidal component, with polyvinylpyrrolidone (Povidone), a synthetic polymer. http://www.ncbi.nlm.nih.gov/pubmed/9474112

Materials and Methods 1) Step 1 – Collecting and growing a sample bacterial contaminant found in the local environment. a) Materials: i) Sterile cotton swabs. ii) Tube of sterile distilled water. iii) Petri dishes with nutrient agar iv) China Marker v) Object to be tested (all are from the front of the class room): (1) Teachers Chair (2) Computer Keyboard (3) Computer Mouse (4) Teachers Desktop b) Method: vi) draw, with a china marker, on the outside of the agar portion, not the lid, of a Petri



References: Short Section Used to list works of others used in the writing of this report. The purpose of this section is to give credit to those whose work was used to test the hypothesis.

You May Also Find These Documents Helpful

  • Powerful Essays

    Obtain two nutrient agar plates and using a permanent marker draw four quadrants on the bottom of each agar plate. Using a sterile pipet transfer 250 ml of E. coli broth to the middle of each petri dish and evenly spread bacterial culture around the agar plate. Cover and allow the culture to soak into agar for at east 15 minutes. Using sterile forceps, carefully place one filter disk from designated sample into the middle of each…

    • 939 Words
    • 4 Pages
    Powerful Essays
  • Good Essays

    Read section II of chapter 26 in your lecture textbook for background on chemical antimicrobial control (for background)—pages 762-763.…

    • 1106 Words
    • 5 Pages
    Good Essays
  • Good Essays

    Ebt1 Task 3 Essay Example

    • 1831 Words
    • 8 Pages

    When a patient is getting ready for surgery, one of the last things on their mind is infection. As medical personnel though, it is a thought that definitely does not leave the mind. Getting an infection after surgery not only increases a patients hospital stay and recovery time, but also increases expenses for the hospital. When thinking of ways to decrease infection, it most often starts with preparation of the surgical site and insertion of invasive catheters. When cleaning the site, many institutions continue to use beta dine though research shows that Chlorhexidine has better results. Though Beta dine and Chlorhexidine both reduce bacterial count, Chlorhexidine is found to be more profound and longer lasting (Jarral, McCormack, Ibrahim & Shipolini, 2011).…

    • 1831 Words
    • 8 Pages
    Good Essays
  • Good Essays

    This experiment is to determine whether toothpaste is more effective at killing the bacteria in ones’ mouth vs the effectiveness of mouthwash at killing bacteria in one’s mouth. This experiment is important for many reasons,…

    • 1333 Words
    • 6 Pages
    Good Essays
  • Powerful Essays

    WHO (2002) antimicrobial (will slip through our grasp says WHO) The Pharmaceutical Journal 264 (7101) pp 902…

    • 6153 Words
    • 25 Pages
    Powerful Essays
  • Best Essays

    Sadowski,, S. (2010). Efficacy of cleaning products for c difficile. PubMed Central (PMC), 56(5), 417-423. Retrieved from…

    • 540 Words
    • 3 Pages
    Best Essays
  • Good Essays

    • Mouth rinses containing the antibiotic medicine may be prescribed. They may lessen symptoms and speed healing.…

    • 457 Words
    • 2 Pages
    Good Essays
  • Powerful Essays

    MICRO BIOLOGY

    • 1277 Words
    • 6 Pages

    For class data there was four organisms investigated which were: Staphylococcus Aureus, Enterococcus hirae, Pseudomonas aeruginosa and Escherichia coli. In general the disinfectant didn’t work as class data shows some organism values weren’t above the benchmark value of 105.…

    • 1277 Words
    • 6 Pages
    Powerful Essays
  • Powerful Essays

    Unit 4222 616

    • 1668 Words
    • 6 Pages

    Antibiotic - To treat infection. Can be specific or broad spectrum. Amoxicillin. Penicillin. Oxycycline. Trimethoprim.…

    • 1668 Words
    • 6 Pages
    Powerful Essays
  • Good Essays

    Essay On Diarrhoea

    • 789 Words
    • 4 Pages

    Transmission could also be reduced by providing separate room and toilet facilities or placing infected patients together. Staff, patients and visitors should wash their hands with soap water regularly and thoroughly; alcohol hand gel should not be used as this has been proven ineffective against the bacteria. Infected surfaces should also be cleaned thoroughly with water and a cleaning product containing bleach. Doctors are advised to prescribe antibiotics cautiously to reduce the broad- spectrum antibiotics given so that the number of people at risk is…

    • 789 Words
    • 4 Pages
    Good Essays
  • Better Essays

    The fact that each bacterial species, and even some of their respective strains, responds uniquely to a given antimicrobial makes it necessary to have methods, which provide researchers and clinicians with measurable susceptibilities. This need has become prevalent since the rising of later generations of antimicrobial compounds achieved by chemical modifications of the naturally occurring antibiotics. By utilizing the Kirby-Bauer method, researchers and clinicians are able to determine the susceptibility of given microorganisms to different antibiotics and antimicrobials. While antibiotics specifically target bacteria, antimicrobials cast a wide net, nonspecifically targeting microorganisms and viruses. During this lab, we tested the effects of both antimicrobials and antibiotics on different bacterial cultures. By creating different lawns of bacteria and placing on each of them disks soaked in different antibiotics and antimicrobials, we were able to observe the susceptibility of the differing bacteria to several agents. This process that we practiced is the Kirby-Bauer method. As stated before, antibiotics and antimicrobials serve differing purposes. Antibiotics are substances, usually in the form of medication, that destroy bacteria or inhibit the growth of bacteria. On the other hand, antimicrobials act in destroying or inhibiting the growth of all microorganisms. One type of antimicrobial is an antiseptic, which acts to reduce the presence of microorganisms without harming living animal tissue (El-Mayas, 2013). It is important to note that while we were able to measure the susceptibility of these agents, we cannot conclude that resistance is present because further investigations would have to be conducted to determine if the microorganism has developed a metabolic capability to withstand the agent’s mechanisms and effects (Maxwell, 2013).…

    • 977 Words
    • 4 Pages
    Better Essays
  • Better Essays

    “In the United States, hospital patients get nearly 2 million infections each year. That’s about 1 infection for every 20 patients. Hand hygiene is one of the most important ways to prevent the spread of infections (Hand Hygiene in Healthcare Settings 2012)” and promote the safety of our patents.…

    • 567 Words
    • 3 Pages
    Better Essays
  • Good Essays

    Cleanliness Champions

    • 562 Words
    • 3 Pages

    With the outbreak of antibiotic resilient infections, infection control is becoming a major concern for health groups all over the world (WHO, 2011). The risk of infection can be reduced by using three types of hand washing known as social, hygienic and surgical methods (HAI, 2011).…

    • 562 Words
    • 3 Pages
    Good Essays
  • Good Essays

    For that purpose, antimicrobial agents such as rifabutin and clarithromycin are most often used, based on the results of retrospective studies and controlled trials that noted lower incidence of the disease in treated groups when compared to placebo groups.…

    • 568 Words
    • 3 Pages
    Good Essays
  • Powerful Essays

    Slides - Drugs ver 1

    • 1565 Words
    • 7 Pages

    Several dyes were found to counteract gram-positive bacterial infections. Prontosil NH2 O H2 N N N S NH2 O Prontosil…

    • 1565 Words
    • 7 Pages
    Powerful Essays

Related Topics