The absorbance of a small test tube filled with 4.0 mL of the 0.2 M NaOH solution and 1 drop of phenolphthalein was recorded every 5 seconds for 360 seconds. Then, ln(absorbance) and 1/(absorbance) were calculated for these values…
Bromothymol blue indicator changes color depending on the pH of a solution. The indicator would change from blue to green to yellow. Using this indicator, we were able to measure the rate of formation of HCl by measuring the time in which the indicator turned blue to green and the amount of NaOH needed to turn it from green to blue again. The time and volume would then be used to obtain the rate constants. Bromothymol blue is a weak acid so it can be protonated and deprotonated causing it to appear yellow and blue.…
Different amounts of FD&C Blue I were diluted with water to make eight differently concentrated 10 mL solutions. Samples were placed in the spectrophotometer to determine the percent transmittance of FD&C Blue 1. All of the data was summarized in graphs to predict the concentration of FD&C Blue I in a sample of Gatorade.…
In order to create a standard curve we created 5 dilutions of solutions containing red dye #40. Concentrations of .666 ug/mL, 1.667ug/mL, 3.333ug/mL, 13.333ug/mL, and 20.000 were measured in a spectrophotometer set to a wavelength of 504 nm to find the amount of light they absorbed. Using Microsoft Excel, we entered the absorbance values as the Y coordinates and the concentrations as the X coordinates to make a line of best fit and that was our standard curve.…
F. Bromothymol blue is blue in the presence of basic solutions, and yellow in the presence of acidic solutions. If your solution is a murky green, what might you assume about the solution?…
chemical Brite, It was observed that the Bromthymol Blue changed to a green color that was not…
In order to complete the objective, the group used a Carolina Digital Spectrophotometer, unit M 201-1, which measures the quantity of light that solutions absorb. The spectrophotometer consists of a light source that shines through a prism which then shines through a…
When Hydrochloric acid and Bromothymal blue are combined there is a physical change. The combination results in a yellowish tint. This means the byproduct of the two is a base.…
This investigation used spectroscopy to evaluate light absorption in different solutions. A spectrophotometer was used in the lab to determine these values. A spectrophotometer is an apparatus used to “measure the absorption of radiation in the visible and UV regions of the spectrum and allows precise at a particular wave length” (Jones et al., 2007). The amount of light absorbed by a substance is directly in relation to the concentration of the solute and also the wavelength moving through the solute (Jones et al., 2007). This is commonly referred to as Beer’s Law and can be expressed as A= εl [C]. Beer’s Law equation measures the absorbency of light, making it an effective measure as spectrophotometers give exact values for absorbency (Jones et al., 2007).…
This lab will teach me how to use a spectrophotometer. The use of the spectrophotometer is to measure the concentration of solute. The solute being measured must be colored and is determined based on the adsorption of light photons on a wavelength. The spectrophotometer uses a beam of light that strikes the diffraction grating that basically forms of prism of light. Then only a specific wavelength of light shines through the spectrophotometer and interacts with the solute. The light that continues past the solute hits the phototube. The spectrophotometer then digitally shows the amount of units that have been absorbed or transmitted. Transmittance is the amount of light that gets through the sample. This is shown as a percent of all the possible light that could’ve gotten through. Absorbance is the opposite of transmittance and the reciprocal of it. This shows how much light got trapped in the solute. In this lab we will use a few different solutions in the spectrophotometer to get a basic feel on how it works. We will record the absorbance as well as do calculations using mean and standard deviation. We will then graph our results and compare them with the class values of the three unknown Methylene blue samples. At the end of the lab the actual concentrations of each unknown will be shared. We will then compare how accurate and precise our results are with the actual.This lab will teach me how to use a spectrophotometer. The use of the spectrophotometer is to measure the concentration of solute. The solute being measured must be colored and is determined based on the adsorption of light photons on a wavelength. The spectrophotometer uses a beam of light that strikes the diffraction grating that basically forms of prism of light. Then only a specific wavelength of light shines through the spectrophotometer and interacts with the solute. The light that continues past the solute hits the phototube. The spectrophotometer…
Blue light cannot be used to measure absorbance of the solution because it cannot be absorbed. Instead of absorbing, the solution reflects blue light…
The disappearance of the purple color indicated the consumption of the crystal violet dye. As the reaction proceeded, the color would fade and more of the incident light would be allowed through. In this experiment the percent transmittance increased as time passed by. The percent transmittance was converted to absorbance, and it is directly proportional to concentration, and our data sets were of absorbance versus time. The abrosbances were treated as if they were actually concentrations of the crystal violet…
To prepare the solution, 4 mL of 80% acetone was pipetted into a test tube, and five drops of the top layer of the pigment extract from the first experiment was combined with the acetone. The test tube was inverted until thoroughly mixed. Then a test tube containing 4 mL of 80% acetone was created to blank the spectrophotometer at 380 nm. The absorbance of the tube with the extract and acetone solution was then tested for its absorbency. These tests were repeated with the same solution at 40 nm intervals, until the wavelength reached 740 nm, re-blanking the machine between each test or change in wavelength. All the absorbencies were recorded, and the areas with the highest absorbance were retested at 5 nm intervals. The results were then plotted on Excel to show the relationship between certain wavelengths and the absorbance of the…
E. Bromothymol blue is blue in the presence of basic solutions, and yellow in the presence of acidic solutions. If your solution is a murky green, what might you assume about a solution?…
The objectives of this experiment are to understand why and how spectroscopy is used to determine the components and concentrations of a solution, describe various types of spectroscopy, describe the visible and ultraviolet regions of the electromagnetic spectrum, define Beer’s law and define the relationship between absorbance and transmittance. Other learning objectives are to create a Beer’s law plot for a series of samples with known concentrations, collect spectrophotomic data from unknown and known FDC blue dye samples, perform serial dilutions, calculate concentrations, perform linear regression and determine the equation of a best fit line.…