Testing catalase activity
Testing Catalase Activity Prepared by Anel Behric
For professor Goodrow
General Biology Lab
Mohawk Valley Community College
10/14/2014 6:00 PM
Introduction: This experiment was conducted to examine the breakdown of substrate hydrogen peroxide (H2O2) by catalase, which is a specific enzyme that breaks down substrates of (H2O2). Molecules are in our bodies and nature. They move around constantly which causes them to be part of random collisions, which produce chemical reactions. This is actually needed to sustain life. These collisions are influenced by many different physical factors, such as temperature or concentration. If more molecules are concentrated in a place, the more random collisions are more likely to happen. If temperature is higher the reaction is sped up, thus leading to more collisions. Enzymes are catalyst because they speed up reaction rates. They are shaped a specific way, so when they collide they fit perfectly into one another. Enzymes are proteins that speed up chemical reactions by decreasing the energy of activation. Enzyme catalase convert hydrogen peroxide in bodies into oxygen and water. This can be visually seen when hydrogen peroxide mixed, and the peroxide bubbles. Catalyst is material that attracts molecules, this increases chances of a collision.
Materials used for this experiment:
LoggerPro pH buffers
Vernier O2 Sensor enzyme suspension
10 mL graduated cylinder test tubes
Ice Pack heating pad
250 mL Nalgene bottle thermometer Three test tubes were placed in a holding rack and labeled accordingly. Each test tube was filled with 5 mL of 3% of H2O2 and 5 mL of pure water. Each tube was filled using a disposable pipette with 10 drops of enzymes suspension to Nalgene chamber for each observation. Tube one was poured into Nalgene chamber, and O2 Gas Sensor was placed on top, and chamber was swirled to mix the enzymes. Same steps were performed with tube
For professor Goodrow
General Biology Lab
Mohawk Valley Community College
10/14/2014 6:00 PM
Introduction: This experiment was conducted to examine the breakdown of substrate hydrogen peroxide (H2O2) by catalase, which is a specific enzyme that breaks down substrates of (H2O2). Molecules are in our bodies and nature. They move around constantly which causes them to be part of random collisions, which produce chemical reactions. This is actually needed to sustain life. These collisions are influenced by many different physical factors, such as temperature or concentration. If more molecules are concentrated in a place, the more random collisions are more likely to happen. If temperature is higher the reaction is sped up, thus leading to more collisions. Enzymes are catalyst because they speed up reaction rates. They are shaped a specific way, so when they collide they fit perfectly into one another. Enzymes are proteins that speed up chemical reactions by decreasing the energy of activation. Enzyme catalase convert hydrogen peroxide in bodies into oxygen and water. This can be visually seen when hydrogen peroxide mixed, and the peroxide bubbles. Catalyst is material that attracts molecules, this increases chances of a collision.
Materials used for this experiment:
LoggerPro pH buffers
Vernier O2 Sensor enzyme suspension
10 mL graduated cylinder test tubes
Ice Pack heating pad
250 mL Nalgene bottle thermometer Three test tubes were placed in a holding rack and labeled accordingly. Each test tube was filled with 5 mL of 3% of H2O2 and 5 mL of pure water. Each tube was filled using a disposable pipette with 10 drops of enzymes suspension to Nalgene chamber for each observation. Tube one was poured into Nalgene chamber, and O2 Gas Sensor was placed on top, and chamber was swirled to mix the enzymes. Same steps were performed with tube
Cited: http://www.novasep.com/misc/glossary.asp?defId=49. (Novasep.) "Enzymes." Enzymes. Biocatalysts: Definition of Biocatalysts in Novasep Glossary." Biocatalysis: Definition of Biocatalysts in Novasep Glossary. http://www.tuberose.com/Enzymes.html>. (Enzymes.) "Frequently Asked Questions http://hyperphysics.phy-astr.gsu.edu/hbase/chemical/ph.html http://www.houston-enzymes.com/learn/faq.php. (Huston.) Jubenville, Robert B., and Richard G. Thomas