(Science Investigation Research)
1 YR – ST. LOUIS OF BERTRAND
SUMITTED TO: Leah Ilagan
(Integrated Science Teacher)
SUBMMITED BY:
Agustin, Nathan Raphael DL.
Arcaira, John Carlo B.
Catuira, Jerrico B.
Olalia, Nicolas Andrei
Serna, Christian O.
Zipagang, Lance Harvey O.
PROBLEM:
How Caffeine affects a Gold-fish
Chapter 1:
The Problem and Its Background
* Background of the Study
* Caffeine is becoming a common chemical found in the environment but, little research has been done to understand the environmental effects of caffeine, including dissolved caffeine in aquatic systems. The goal of this research study was to begin to understand how caffeine may interact with aquatic environment, using the bacteria of the genus Pseudomonas, commonly found in the aquatic habitat. We found that Pseudomonas shows an increase in growth when exposed to caffeine, which results in a change in spectrophotometric absorbance, increase in bacteria colony count in the presence of caffeine, and in bio-film like sheen appearing on the glass of the experimental aquarium. Along with the increased bacterial growth, a dramatic increase in ammonia concentration was observed. Ammonia is toxic to fish, and can be correlated with the metabolic activity of the Pseudomonas bacteria, making the caffeinated environment toxic for aquatic life.
* Statement of the Problem
* The statement tells us how those caffeine affects any factors of the fish, on its behavior and its body
* The Hypothesis of the Study
* If caffeine has been presented in an aquatic environment, then the living-things in that surrounding area had a mysterious reaction because caffeine acts us an energy giving nutrient in a human body, making other things more hyper active.
* Significance of the Study
* It makes us know how caffeine affects are body and how much boost it gives us in every beverage we take with it. Coffee contains more than