The New D-Dimer Assay
UNIVERSITY OF HULL
MODULE NO: 58372
Laboratory based skills 4
Noel Bacasmas
Student no: 201012397
BSc Applied Biomedical Science
(Part time)
Contents Page No.
Title and learning objectives.....................................................................3
Introduction.............................................................................................5
Mechanisms of Fibrinolysis.......................................................................6
Different methodologies/Assays to Detect Fibrinolysis...................,,.......12
Venous Thromboembolism; Pathophysiology, Clinical features and Diagnosis.....................................................15
Materials and Methods...........................................................................20
Results.....................................................................................................22
Discussions..............................................................................................23
Conclusion...............................................................................................23
References..............................................................................................25
Appendices.............................................................................................26
LEARNING CONTRACT
TITLE OF LEARNING CONTRACT:
D-dimer HS, a new highly specific quantitative assay for D-dimer testing.
LEARNING OUTCOMES: * Make a logical presentation of the learning contract. * Research relevant literatures from textbooks, scientific journals, standard operating procedures in the laboratory, manufacturer’s manuals and internet website. * Evaluate and validate the performance of the new test kit D-dimer HS using the ACL TOP analyzer by comparing it with the Hemosil D-dimer assay the current kit SGH is using. * Gain knowledge and
MODULE NO: 58372
Laboratory based skills 4
Noel Bacasmas
Student no: 201012397
BSc Applied Biomedical Science
(Part time)
Contents Page No.
Title and learning objectives.....................................................................3
Introduction.............................................................................................5
Mechanisms of Fibrinolysis.......................................................................6
Different methodologies/Assays to Detect Fibrinolysis...................,,.......12
Venous Thromboembolism; Pathophysiology, Clinical features and Diagnosis.....................................................15
Materials and Methods...........................................................................20
Results.....................................................................................................22
Discussions..............................................................................................23
Conclusion...............................................................................................23
References..............................................................................................25
Appendices.............................................................................................26
LEARNING CONTRACT
TITLE OF LEARNING CONTRACT:
D-dimer HS, a new highly specific quantitative assay for D-dimer testing.
LEARNING OUTCOMES: * Make a logical presentation of the learning contract. * Research relevant literatures from textbooks, scientific journals, standard operating procedures in the laboratory, manufacturer’s manuals and internet website. * Evaluate and validate the performance of the new test kit D-dimer HS using the ACL TOP analyzer by comparing it with the Hemosil D-dimer assay the current kit SGH is using. * Gain knowledge and
References: Buetler,E., Lichtman, M., Coller, B. And Kipps, T. (1995) Williams Hematology,5th Edition,New York USA, McGraw Hill publishers. Colman,R., Hirsh,J.,Marder,V. And Salzman,E.(1994) Hemostasis and Thrombosis, Basic Principles and Clinical Practice, 3rd Edition, Pennsylvania USA,J.B.Lippincott publishers. Courtney, M., Steinberg, J.and McCormick, J.(2010) Prospective diagnostic accuracy assessment of the Hemosil HS D-dimer to exclude pulmonary embolism in emergency department patients. Thrombosis Research 125, 79-83. De Moerloose,P., Vanrusselt, M.,Reber,G. and Arnout, J. (2005) Performances of the Hemosil D-dimer HS assay for the exclusion of venous thromboembolism. Journal of Thrombosis and Haemostasis 3,2361-2363. Ghanima,W. and Sandset,P.M.(2007) Validation of a new D-dimer microparticle enzyme immunoassay (AxSYM D-dimner) in patients with suspected pulmonary embolism. Thrombosis Research 120,471-476. Hirsh,J. And Lee, Y.A.(2002) How we diagnose and treat deep vein thrombosis. Blood 99, 3102-3110. Lip, G.Y.H. and Blann, A. (2003) ABC of Antithrombotic Therapy, London UK,BMJ publishing group. Moresco, R.N., Vargas,L.C.R. and Silla, L.(2007) Estimation of the levels of D-dimer by use of an alternative method based in the reaction time of fibrinogen/fibrin degradation products assay. Journal of Thrombosis and Thrombolysis 24,73-76.