Thermostable Β-Macromidase Enzyme
With the advances in technology, aging of the population and the steady progress in the field of biological sciences, treatment of diseases and protection against pathogens have become more complex than ever. Technology sheds light on how to treat diseases, thus different quantitative experimental techniques and mathematical analyses for improving biological research are available, yet healthcare costs are skyrocketing, and the knowledge on how macromolecules work and how cells regulate gene expression has been a quandary. Biology is a highly fragmented science with delicate details at all levels where macromolecules perform a wide array of functions necessary for the survival and growth of living organisms and hence important cellular components.
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My graduate studies dealt with extensive and comprehensive advanced coursework and research, which not only helped me learn and understand the intricacies of Microbiology and Molecular Biology, but also provided an insight of its association with other fields like Biochemistry and Bioinformatics. I completed my graduate research work under the guidance of Dr. Shaikh, who is an expert in the field of Enzymology and Virology. The objective of my thesis was to produce and characterize thermostable β-Galactosidase enzyme, which has been explored in various biotechnological, industrial, medical and analytical applications. The focus of research was not only limited to thermo stability of β-Galactosidase, but also to the production of large amount of enzyme in a short period of time. The β-Galactosidase enzyme hydrolyzes lactose into galactooligosaccharides, which are suitable for lactose-intolerant people and has a positive effect on the intestinal bacterial microflora. While conducting my graduate research, I worked on different biophysical techniques such as gel electrophoresis, colorimetry and UV Spectroscopy. The applicability of instruments, such as high speed and ultra-speed centrifuge, one-dimensional and two-dimensional electrophoresis, Quebec Colony Counter, and quantitative PCR had been a significant part of my research during my graduate studies and the knowledge of these important techniques will surely prove valuable and assist me in further exploring the field of biochemistry and
My graduate studies dealt with extensive and comprehensive advanced coursework and research, which not only helped me learn and understand the intricacies of Microbiology and Molecular Biology, but also provided an insight of its association with other fields like Biochemistry and Bioinformatics. I completed my graduate research work under the guidance of Dr. Shaikh, who is an expert in the field of Enzymology and Virology. The objective of my thesis was to produce and characterize thermostable β-Galactosidase enzyme, which has been explored in various biotechnological, industrial, medical and analytical applications. The focus of research was not only limited to thermo stability of β-Galactosidase, but also to the production of large amount of enzyme in a short period of time. The β-Galactosidase enzyme hydrolyzes lactose into galactooligosaccharides, which are suitable for lactose-intolerant people and has a positive effect on the intestinal bacterial microflora. While conducting my graduate research, I worked on different biophysical techniques such as gel electrophoresis, colorimetry and UV Spectroscopy. The applicability of instruments, such as high speed and ultra-speed centrifuge, one-dimensional and two-dimensional electrophoresis, Quebec Colony Counter, and quantitative PCR had been a significant part of my research during my graduate studies and the knowledge of these important techniques will surely prove valuable and assist me in further exploring the field of biochemistry and