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Thin-Layer Chromatographic Analysis of Drug Components

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Thin-Layer Chromatographic Analysis of Drug Components
EXPERIMENT 15

Thin-Layer Chromatographic Analysis of Drug Components

Experiment Objective:
To identify the components of an analgesic drug tablet and then correctly identify the tablet from a group of others with acquired data.

Experiment Summary:
In this experiment, we use TLC to identify components of an unknown analgesic drug. We prepare a solution of the drug by dissolving part of a tablet in 1:1 ethanol/dichloromethane, then spotting a TLC plate with the solution along with standard solutions of all active substances the drug tablet is likely to contain. Once the TLC plate develops and dries, it is viewed under ultraviolet light to properly visualize and mark the greatest intensity of spotting.

Experiment Data:

Rf Value = distance from baseline travelled by solute/distance from baseline travelled by solvent
Unknown =

Trial 1:
Rf1 Unknown 5.1cm/7.4cm = .689
Rf1 Unknown 5.0cm/7.4cm = .676
Rf2 Aspirin 5.0cm/7.4cm = .676
Rf3 Acetaminophen 2.9cm/7.4cm = .392
Rf4 Ibuprofen 2.9cm/7.4cm = .392
Rf5 Salicylamide 5.0cm/7.4cm = .676
Rf6 Caffeine 1.0cm/7.4cm = .135

Trial 2:
Rf1 Unknown 5.0cm/7.4cm = .676
Rf1 Unknown 5.0cm/7.4cm = .676
Rf2 Aspirin 5.2cm/7.4cm = .703
Rf3 Acetaminophen 3.3cm/7.4cm = .446
Rf4 Ibuprofen 4.9cm/7.4cm = .662
Rf5 Salicylamide 5.1cm/7.4cm = .689
Rf6 Caffeine 0.9cm/7.4cm = .122

Observations, Results, & Discussion:
After performing the experiment twice to improve the accuracy of our findings, we were still left somewhat unsure with our outcome. After computing the Rf values for each of our trials, our final calculations did not leave us with clear cut and easily discernable results. Overall, between the two separate trials, we spotted and calculated our knowns twice each, and our
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